細胞外マトリックスを応用した新しい歯科治療法の可能性について(<特集>歯科における結合組織研究の進歩)

概要

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The regeneration of teeth and bone becomes to be a new therapy in the near future. Our group have been proposed that the extracellular matrix is an powerful stimulator for the regeneration of tissues. Recently we found that bone marrow cells which were harvested from rat femur, differentiated into osteoblasts when cells were cultured with type I collagen. Type II, III, and V collagen did not show the inducing activity. Therefore the inducing activity of type I collagen is specific. The induction by collagen was mediated by α2β1 integrin receptor of cells. The osteoblastic differentiation was inhibited by the interruption of the binding of collagen with α2β1 integrin. Type I collagen contributes to the osteoblastic differentiation at the early stage. At the late stage, bone sialoprotein(BSP)had a crucial role for the differentiation. When the binding of BSP with receptor was suppressed by the presence of the specific antibody, the expression of osteoblastic phenotypes of bone marrow cells were suppressed. Type I collagen enhanced the formation of mineralized tissues by dental pulp cells in the medium containing β-glycerophosphate. These findings imply that extracellular matrix facilitates the formation of mineralized tissues.
日本結合組織学会の論文
2000-03-25