A Novel Tool of Temperature-Responsive Cell Culture Surfaces and Its Application to Matrix Biology

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概要

• 論文の詳細を見る

• Matrix biology has one characteristic feature which is not covered by traditional biochemistry or molecular biology. Matrix biology inevitably includes solid materials for the alignment of cells in tissue architectures. One critical problem to overcome for elucidation of the consolidated structure and function is limitation in solubilization of the extracellular matrix(ECM)components. In this minireview, we introduce a unique novel cell culture dish developed by us. The dish surface shows similar hydrophobicity as normal polystyrene tissue culture dishes at 37°C. When the temperature is reduced below 32°C, the surface rapidly hydrates and becomes hydrophilic. Therefore, by only reducing temperature viable cultured cells and intact deposited ECM are recovered from the temerature-responsive culture dish surface with no requirement of digestive enzymes or denaturing treatments. The possibility that cell culture system utilizing this unique novel culture surface provides a useful tool to investigate solid ECM is discussed.

• 日本結合組織学会の論文
• 1999-03-25

著者

• Kikuchi Akihiko

  Department Of Electrical And Electronics Engineering Sophia University

• Kikuchi A

  Institute Of Advanced Biomedical Engineering And Science Tokyo Women's Medical University

• SAKURAI Yasuhisa

  Institute of Biomedical Engineering, Tokyo Women's Medical University

• Okano Teruo

  大阪大学 医学系研究科心臓血管外科

• Okano T

  Institute Of Advanced Biomedical Engineering And Science Tokyo Women's Medical University

• Okano Teruo

  Institute Of Advanced Biomedical Engineering And Science Tokyo Women's Medical University

• YAMATO Masayuki

  Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University

• KIKUCHI Akihiko

  Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University

• KUSHIDA Ai

  Institute of Biomedical Engineering, Tokyo Women's Medical University, Tokyo, Japan

• KONNO Chie

  Institute of Biomedical Engineering, Tokyo Women's Medical University, Tokyo, Japan

• Sakurai Y

  Institute Of Biomedical Engineering Tokyo Women's Medical University

• Sakurai Yasuhisa

  Department Of Materials Science And Research Institute For Biosciences Science University Of Tokyo

• Yamato Masayuki

  Institute Of Advanced Biomedical Engineering And Science Tokyo Women's Medical University

• Yamato Masayuki

  Department Of Physics Faculty Of Science Tohoku University:nippon Kokan K.k. Technical Research Cent

• Konno Chie

  Institute Of Biomedical Engineering Tokyo Women's Medical University Tokyo Japan

• Kikuchi A

  Institute Of Advanced Biomedical Engineering And Science Center Of Excellence (coe) Program For The

• Sakurai Yasuhisa

  Institute Of Biomedical Engineering Tokyo Women's Medical University

• Kushida Ai

  Institute Of Advanced Biomedical Engineering And Science Center Of Excellence (coe) Program For The

• Yoshida Masayuki

  Institute Of Advanced Biomedical Engineering And Science Tokyo Women's Medical University

• Yoshida Miyoko

  Division Of Regenerative Med. Department Of Basic Clinical Science Tokai Univ. School Of Med.

• Okano Teruo

  Institute Of Advanced Biomedical Engineering And Science Center Of Excellence (coe) Program For The

• Yamato Masayuki

  Institute Of Advanced Biochemical Engineering And Science Tokyo Women's Medical University

• Okano Teruo

  Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University

• OKANO Teruo

  Institute of Advanced Biomedical Engineering and Science (TWIns), Tokyo Women's Medical University

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