抗癌剤の投与経路が可移植性ヒト口底癌に及ぼす影響

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The effect of anticancer drugs administered by different routes on human oral cancer was examined. The experimental chemotherapy with 5-fluorouracil (5-FU) and cisplatin (CDDP) given by the three different routes per os, intraperitoneal (i.p.) and dorsal hypodermic (d.h.) was carried out on nude mice bearing human oral cancer. Changes in body weight and relative tumor weight of the animals, and nuclear DNA contents of the cancer cell were examined and histopathological and immunohistochemical observations of the tumor were also carried out. The body weight of the animals hiven a single 5-FU dose significantly decreased when compared to the control from the first day after administration. The weight loss by per os administration was more prominent than that by other routes of administration on the third and the fifth days. The body weight in the combination groups given 5-FU and CDDP was significantly dercreased from the first day after 5-FU administration. Among the three routes, administration by the per os route caused the greatest decrease in weight on the fifth day after 5-FU administration. The relative tumor weight in the single administration group via each route was significantly reduced on the fifth day and significantly different on the sixth day between i.p. and d.h.administration. In the combination groups the relative tumor weight was significantkly reduced from the second day after 5-FU administration, among which the i.p.group showed the greatest reduction up to the fifth day. The tumor in each group was subsquently extirpated and microscopically observed. According to Simosato's classification, the tumors in the single 5-FU administration group were grade II a, those in the combinaiton groups by per os and d.h.administration were grade II a and those by i.p.administration grade II b+III. The lowest ratio of the proliferating cell nuclear antigen (PCNA) positive cell population, was obtained by i. p. administration both in the single and combination administration groups. Changes in each phase cell population after chemotherapy were as follow : In the single per os administration the G_0/G_1 phase cell population significantly decreased on the first, third and fifth day, the S phase cell population significantly increased and the G_2+M phase cell population showed no significant change. The G_0/G_1 phase cell population significantly decreased on the third day after i.p. administration, the S phase cell population significantly increased on the third and fifth days and the G_2+M phase cell population on the sixth day. The G_0/G_1 phase cell population significantly decreased after d.h.administration on the fifth day, whereas the S phase cell population significantly increased on the fifth day and the G_2+M phase cell population on the sixth day. In the CDDP one-shot group, the G_0+G_1 phase cell population significantly decreased on the first day, whereas the S and G_2+M phases significantly increased on the first and second days. In the 5-FU one-shot group there was no significant change. In the combination group the G_0+G_1 phase cell population significantly decreased from the first day after per os administration of 5-FU, whereas the S phase cell population significantly increased from the first day and the G_2+M phase cell population from the second day. From the first day after the i.p.administration of 5-FU in the combination group, the G_0+G_1 phase cell population significantly decreased, whereas the S phase cell population significantly increased from the first day and the G_2+M phase cell population increased on the first, second, fourth and fifth day. The G_0+G_1 phase cell population significantly decreased from the first day after d.h.administration of 5-FU in the combination group, whereas the S phase significantly increased from the first day, but the G_2+M phase cell population showed no significant change on any experimental day.
福岡歯科大学学会の論文
1995-09-30

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