化学的交感神経遮断下のラット顎下腺の分泌応答

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The submandibular glands (SMG) of rats can discriminate the nature of the stimulus and secrete three types of proteins in response to various sialogogues, as termed α, β and γ by us. Little information, however, is available on the secretory functions of the SMG of rats under chemical sympathectomy. This study was thus designed to determine whether five kinds of receptors existed in the SMG changed their secretory responses, three phenolamines and dopamine acted indirectly or directly and the secretory functions of the SMG subjected to the repeated amputation of lower incisor teeth (AMP) were changed under the chemical sympathectomy induced by reserpine (Res) or 6 hydroxydopamine (6-OHDA) for 7 days with and without various kinds of agonists and antagonists, by using Lowry's method, enzyme immunoassay, various electrophoreses and histology. The results were as follows : 1. Wet weights of the SMG were significantly increased at 1 and 2 days, whereas body weight and wet weights of the sublingual gland (SLG) were significantly reduced at 7 days after Res treatment. 2. Wet weights/body weight of the SMG and SLG were significantly reduced at 1 day after Res treatment in combination with pargyline, a monoamine oxidase inhibitor. 3. Enlargement of the acinar cells and atrophy of granular convoluted tubule cells were histologically observed in the SMG at 7 days after Res treatment. 4. Supersensitivities to adrenergic β_1-and α_2-, peptidergic, bioaminergic and cholinergic receptors were observed in the SMG after Res treatment. Especially, supersensitivity was dramatically elucidated in the SMG after Res treatment in response to noradrenaline. 5. Salivation by the SMG after Res treatment was completely abolished in response to tyramine. Partial desensitization was occurred in the SMG after Res treatment in response to p-octopamine, m-octopamine and dopamine depending upon doses used. 6. Salivation in response to p-octopamine was significantly reduced in the SMG after Res treatment with an adrenergic α_1-selective blocker, but not with other antagonists, namely propranolol, atropine and a calcium ion channel blocker. 7. Supersensitivities to the α-type proteins secreted by the SMG after Res treatment were dramatically occurred in response to noradrenaline, but not to dopamine and p-octopamine at low doses used. 8. Body weight reduced significantly by Amp alone was significantly less than that with Amp alone in combination with Amp and Res or 6-OHDA at 7 days after treatment. 9. Wet weights/body weight of the SMG and SLG were significantly increased at 7 days after Amp alone, whereas of the SMG, but not of the SLG, they were much more significantly enlarged with Amp and Res, but not with Amp and 6-OHDA, treatment than those with Amp alone. 10. Secretory responses of the SMG with Amp alone and Amp with Res treatment were very similar but greatly different between Amp alone and Amp with 6-OHDA treatment. No changes were observed in the types of proteins in response to p-octopamine with Amp in combination with Res or 6-OHDA treatment.
福岡歯科大学学会の論文
1994-06-30

化学的交感神経遮断下のラット顎下腺の分泌応答

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