鶏血漿中 Aspartate-および Alanine-aminotransferase 系アミノ基転移酵素の分別法について

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In purifying the transaminase in fowl blood plasma, it is necessary to use a methodfor systematic separation of the blood plasma. No detailed investigations on this aspecthave been reported. Then, during the days previous to the subfractionation of plasmaprotein components, the plasma was separated by means of successive fractional precipi-tation at a controlled pH value. The isoelectric point of transaminase in the fowl bloodplasma was determined as follows.In addition, the aspartate aminotransferase GOT) and alanine aminotransferase(GPT) reported in this paper could be classified into four types known by the generalterms F-GOT, R-GOT, F-GPT, and R-GPT. These terms were used as synonyms offorward-GOT, reverse-GOT, forward-GPT, and reverse-GPT, respectively. Each of themwas divided by the kind of transaminase that was directly related to the reversible trans-amination reaction.(a) F-GOT: pH 7.2 and 4.9(b) R-GOT: pH 6.9 and 5.2(c) F-GPT: pH 5.8 and 4.6(d) R-GPT : pH 6.9 and 4.6These findings offered helpful suggestions for the fractionation of transaminases bythe use of Cohn and Oncleys methods. Then, of these methods, the 6th and 9th metI?odswere used experimentally for the purpose of fractionating the transaminase. As a result,it was found that the 9th method of ONCLEY was effective for practical purposes. Is-pecially, the fractions of IV-5, 6, 7, and 8 that had been subfractionated from Cohnsfractions.IV-4 by the 9th method of ONCLEY showed activities higlter than the remaining fractions,In those fractions, especially that of IV-5, nothing could comperasate the appearanceof F-GOT and F-CPT activities, but only R-GOT and R-GPT activated the fraction ofIV-5. This result suggests that there may be some correlation concerning the mode offractional precipitation between R-GOT and R-GPT. More detailed information 011this aspect is to be given in a report to come.In this way, each of the active fractions for individual transaminase is no good agree-ment concerning the mode of fractional precipitation.On the basis
社団法人日本獣医学会の論文
1972-02-25

鶏血漿中 Aspartate-および Alanine-aminotransferase 系アミノ基転移酵素の分別法について

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