Atomic Force Microscopy Studies of Erythocyte Membranes Immobilized by Centrifugation under Aqueous Conditions (<Special Issue> Scanning Tunneling Microscopy)

概要

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Structural studies of human erythrocytes and corresponding membranes (erythrocyte ghosts and inside-out vesicles) immobilized by centrifugation under aqueous conditions using atomic force microscopy (AFM) are described. The samples immobilized by centrifugation on mica substrates provided much better images than those simply adsorbed on the substrates, and we obtained images of mesh-like structures and circular, plaque-like structures. Comparison of these structures using electron microscope images reported to date suggests that the observed structures seem to correspond to the cytoskeletal networks and the cell surfaces, respectively. Bundle structures, thought to be induced by the interaction between the AFM tip and the cytoskeletal networks, were occasionally observed. The centrifugal fixation method, which is described here for the first time, can easily immobilize biomembranes under aqueous conditions and is expected to enable the detailed observation of various types of living cells and biomembranes under physiological conditions.
社団法人応用物理学会の論文
1997-06-30