豚肺虫症の免疫に関する研究 : IV. X線照射減弱子虫で免疫した除胸腺モルモットの感染防御に対するX線全身照射ならびに抗胸腺細胞血清投与の影響

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Two experiments, I and II, were carried out on guinea pigs to elucidate the defense mechanism against Metastrongylus apri infection. In Experiment I, to produce the status of scarcencss in thymus-derived cells (T-cells) animals were exposed to whole body X-irradiation (300 R) seven days after thymectomy. Immediately after exposure they were vaccinated orally with 1,000 larvae attenuated by 5×10^4R of X-rays (Group A). On the 25th day after vaccination they were subjected to challenge infection with 500 normal larvae. Two control groups, B and C, were set up. In Group B, animals were not treated, but vaccinated and challenged. In Group C, animals were not treated nor vaccinated, but challenged. All the guinea pigs were killed on the 21st day after challenge to examine the presence of worms in the lungs. The number of worms recovered from the lungs was 0-77 (mean, 22.8) for Group A, 0-2 (mean, 0.3) for Group B and 1-116 (mean, 43.4) for Group C. There was a significant difference in worm burdens, as determined by Mann-Whitney's "U" test, between Groups A and B, but not between Groups A and C (P<0.05). These results showed that T-cells might play an important role in the identification of larval antigen. In Experiment II, thymectomized guinea pigs were vaccinated orally with 1,000 larvae attenuated by 5×10^4R of X-rays. Then 25 days later they were subjected to challenge infection with 500 normal larvae. They were given intraperitoneally with 1.0 ml of antithymocyte serum (ATS) on the day of challenge infection and then with 0.5ml every two days until 9 injections were made (totalling 5.5ml each), so that T-cells might be suppressed (Group A). Two control Groups, B and C, were set up. In Group B, animals were not treated, but vaccinated and challenged. In Group C, animals were not treated nor vaccinated, but challenged. On the 21st day after challenge, all the animals were killed to examine the presence of worms in the lungs. The number of worms recovered from the lungs was 4-254 (mean, 44.7), 0-5 (mean, 1.2) and 29-274 (mean, 95.8) for Groups A, B and C, respectively. There was a significant difference in worm burdens, as determined by Wilcoxon's "U" test, between Groups A and B, as well as between Groups A and C (P<0.05). These results showed that T-cells, which had a close relationship to cell-mediated immunity, might be suppressed by thymectomy and ATS injection, suggesting the important role of these cells in the defense mechanism against M. apri infection.
社団法人日本獣医学会の論文
1976-12-25

豚肺虫症の免疫に関する研究 : IV. X線照射減弱子虫で免疫した除胸腺モルモットの感染防御に対するX線全身照射ならびに抗胸腺細胞血清投与の影響

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