尿中クロムの原子吸光法による測定法とその応用例

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An accurate method for the determination of microamount of chromium in the human urine by atomic absorption spectroscopy is described. And analytical data of 88 human urine samples by this method are shown in this report. About 50ml or 20ml amount of samples is mineralized with nitric acid and sulfuric acid. By reason of taking off the inorganic compound interfering with chromium oxidation, the mineralized residue must be completly decomposed by strong reheating until dense fumes of sulfuric trioxide are carried out. The chromium was oxidized to chromium (VI) with a small amount of 0.01N KMn0_4 solution. Two percent of APDC solution is added to it when the solution pH has been adjusted to 4, and the chromium chelate is extracted into MIBK. The keton extract is aspirated into the acetylene-air flame. Atomic absorption spectroscopy is operated at the highest sensitivity using the 3579Å line of chromium. The limit of detection for chromium was found to be 0.01μg. The recovery of chromium with the entire procedure using the radioactive isotope of chromium was 82.1-94.0 percent. By the aplication of this method, 88 urine samples of 30 persons belonging to three groups (I: chromate producing factory workers, II: the inhabitants in the vicinity of the factory and III: the control) were analyzed. The distribution of frequencies of the analytical date showed positive skewing to cluster near zero, but the distribution of logarithmic values was nearly normal. The representative numerical values of chromium in the urine of these three groups are as follows; 1. Geometric means of the chromium concentration in the urine adjusted for specific gravity of 1.020 were 0.0243 ppm for I group, 0.0030 ppm for II group and 0.0030 ppm for III group. 2. Geometric means of chromium excretion in 8 hours' urine adjusted for creatinine were 19.72μg/g for I group, 2.49μg/g for II group and 2.10μg/g for III group.
社団法人日本産業衛生学会の論文
1971-11-20

尿中クロムの原子吸光法による測定法とその応用例

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