慢性四塩化炭素中毒の血液学的実験研究
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概要
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Hematological studies on the chronic CCl_4 poisoning were performed in thirteen female rabbits, which were divided into five controls and eight CCl_4 injected group. The CCl_4 animals were administered with the poison three times a week by subcutaneous routes in the dose of 0.022 cc CCl_4 per kg. body weight which was determined by the previous study. Three of CCl_4 poisoning group were used for histological observation at about first month from the beginning of the experiment. The examinations were made approximately once a week concerning the following items; body weight, red blood cell and leulocyte count, hemoglobin content, hematocrit value, icteric index, color index, volume index, average diameter of erythrocytes, osmotic fragility of red cells, plasma volume, serum iron level, organ iron contents, weight of organs and microscopic examination of organ tissues. The results obtained were as follows: 1. The average body weight of controls increased gradually more than the CCl_4 group which kept on constant level along the course of the experiment. 2. Red cell count, hemoglobin content, leucocyte count and hematocrit value in CCl_4 group ran parallel with each other, and all of them showed two peaks throughout the course. 3. The icteric index of CCl_4 group was maintained on a higher level than that of controls. In the course of the experiment it was highest in the earlier stage of intoxication. 4. In the poisoned group the average diameter of red cells, color index and volume index were increased. 5. The osmotic fragility of red cells in the CCl_4 group showed a tendency to reduce after two weeks. 6. The plasma volume as well as the blood volume of CCl_4 group had a tendency to increase gradually. 7. The serum iron level in the CCl_4 group increased gradually and reached its highest level at the third week. 8. Evidence of changes in iron content of organs of the CCl_4 group was not found. 9. The liver of poisonned animals gained in weight in the beginning, but decreased at the end. The weights of the poisonned spleen and kindey were increased and that of the poisoned heart decreased. The most characteristic gain of weight, however, was found in the adrenal gland. 10. Concerning microscopic findings of organ tissues, the most marked change was found in the liver, which showed earlier features of cirrhosis during the first month of the poisoning and at the end of the experiment a progressed, definite liver cirrhosis was revealed. The bone marrow tissues were microscopically hypoplastic at the first month, but they became hyperplastic at the end.
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