High resolution physical mapping of genes of plant chromosomes(Symposium : Manipulating Chromosomes)(Abstracts of the 52nd Annual Meeting of the Society of Chromosome Research)

概要

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Fluorescence in situ hybridization (FISH) is an effective method for physical mapping of genes and repetitive DNA sequences on plant chromosomes. Physical mapping of unique nucleotide sequences on specific rice chromosomes was performed by a combination of chromosome identification and high sensitivity FISH. Increase in detection sensitivity of smaller targets and improvements in spatial resolution detection of fluorescent signals, have led to the development of a new phase in FISH technology. Thus, it is now possible to perform in situ hybridization on pachytene chromosomes and extended DNA fibers (EDFs) in rice. EDF-FISH and other DNA combing techniques can provide a 1 kb spatial resolution between adjacent targets, and the detection of even a 300 bp target is now feasible. Such techniques can reveal the spatial organization of the terminal structures of all rice chromosomes. Furthermore, the numbers of copies numbers of various repetitive sequences in rice genome could be determined. Molecular combing techniques are useful tools for measuring the size of various DNA molecules. This review describes the significance of these accurate molecular cytogenetic advancements for plant genetics, and discusses future plant genome studies using visualization techniques.
財団法人染色体学会の論文