Effects of Cytokines Induced by Mineral Dust on Lung Fibroblasts In Vitro
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概要
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Rabbit's alveolar macrophages (AM) obtained by lavage were cultured with three mineral dusts (quartz, asbestos fibre and uranium dust) in vitro. The activity of tumor necrosis factor (TNF) and lung fibroblast (LF) proliferation were measured by ^3H-thymidine (^3H-TdR) incorporation, the collagen synthesis in LF by ^<14>C-proline (^<14>C-Pro), interleukin-6 (lL-6) activity in the supernatant of AM by 3-(4,5-dimethyiazo-2-yl)-2,5-diphenyltetrazolium bromide; thiazoylblue (MTT) colorimetry, and the total hydroxy-proline (HOP) in LF by the chloromine-T method. The assay of the inhibiting effect of anti-TNF antibody and IFNγon LF proliferation and collagen synthesis were carried out. The results show that the three mineral dusts can induce AM to release TNF and IL-6. When the three mineral dusts were added at a dose of 200 μg/ml, the levels of TNF were 1369 U/ml, 1198 U/ml and 852 U/ml, and the levels of IL-6 were 1336U/ml, 1511U/ml and 1335 U/ml, which were significantly higher than those in the TiO_2 control. The LF proliferation and collagen synthesis can be increased by the supernatant of the AM treated with the three mineral dusts. ^3H-TdR incorporations were 22320 dpm, 12547 dpm and 15048 dpm (at a 1:2 dilution of AM supernatant), which was significantly higher than in the TiO_2 and Hank's control (P<0.01). ^<14>C-Pro incorporations were 34001 dpm, 16319 dpm and 22550 dpm (at a dust concentration of 200 μg/ml), which were much higher than in the TiO_2 and Hank's control (P<0.01). Total amounts of HOP in Wl-38 cell activated by supernatants of AM induced by the three mineral dusts were 22.41 μg/ml, 24.00 μg/ml and 21.29 μg/ml(at a dust concentration of 200 pμg/ml), which were significantly higher than in the TiO_2 and Hank's control (P<0.01). Both anti-tumor necrosis antiboby and interferon-gamma can inhibit the proliferation of the LF and decrease collagen synthesis.
- 社団法人日本産業衛生学会の論文
著者
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Zhou Li
Department Of Histology And Embryology Norman Bethune Medical School Of Jilin University
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Zhou Jian
Department Of Occupational Health Suzhou Medical College
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Yang Ji
Department Of Occupational Health Suzhou Medical College
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Zhou Li
Department Of Occupational Health Suzhou Medical College
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Zhou Jian
Department Of Chemistry University Of Science And Technology Of China
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Yang Ji
Department Of Modern Mechanics University Of Science And Technology Of China
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Zhou Li
Department Of Chemistry Huaibei Coal Teachers College
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