Promotion of crown-gall tumor initiation on primary pinto bean leaves by certain inorganic salts

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Crown-gall tumor initiation by Agrobacterium tumefaciens (SMITH and TOWN.) CONN, strain B 6, on Phaseolus vulgaris L. var. "Pinto" was found to be sensitive to the addition of various salts to the freshly inoculated primary leaves. Calcium, magnesium, zinc and ammonium sulfates, cobalt and nickel chlorides and potassium nitrate increased the number of tumors on inoculated leaves by 100 to 200 percent. Maximal promotions were obtained at concentrations of 10^<-7> to 10^<-5> M With all of these salts except potassium nitrate which was most active at 10^<-2> M. Sodium, potassium, calcium, magnesium and aluminum chlorides, and sodium, potassium and nickel sulfates had little or no effect on tumor initiation. The combination of sodium sulfate with magnesium chloride gave promotions comparable to those obtained with magnesium sulfate, indicating that both the magnesium and sulfate ions were necessary for the promotion obtained with this latter salt. Combining any two of the active salts at their optimum concentrations for prpomotion resulted in a reciprocal inhibition of the promotion obtained with either salt alone, suggesting these salts to be active by different and potentially antagonistic mechanisms. The possible nature of these promotions is discussed, but they remain too obscure to warrant a specific proposal for their mode of action.
日本植物生理学会の論文