Synthesis of mRNA is Required for Light-Induced Phase Shifting of the Circadian Conidiation Rhythm in Neurospora crassa : ENVIRONMENTAL AND STRESS RESPONSES

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The process of light-induced phase shifting was investigated in Neurospora crassa using a liquid culture system and a combination of treatment with a nucleoside analogue and light. 5-Azacyiidine inhibited the light-induced phase shifting at all phases that were sensitive to light. Electrophoresis of proteins that were synthesized in a translation system in vitro showed that 5-azacyiidine inhibited the synthesis of most mRNAs. The inhibition of mRNA synthesis was correlated with the inhibition of light-induced phase shifting. An excess of cytidine completely over-came the inhibition by 5-azacyiidine of both light-induced phase shifting and mRNA synthesis. Other analogues, namely, 6-azauridine and 6-methylpurine, failed to inhibit either the light-induced phase shifting or the synthesis of mRNA. Two-dimensional gel electrophoresis showed that the levels of expression of nine mRNAs were affected by light within 30 min after irradiation. By contrast, the oscillator of the circadian clock was not affected by pulse treatment with 5-azacyiidine alone because such treatment failed to shift the phase of the circadian rhythm at any phase. These results indicate that newly synthesized mRNA(s) is required during the process of signal transduction, from the light-perceiving system to the circadian clock, for light-induced phase shifting in Neurospora.
日本植物生理学会の論文