Phosphoglycolate Phosphatase-Deficient Mutants of Chlamydomonas reinhardtii Capable of Growth under Air : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
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概要
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Mutants deficient in phosphoglycolate phosphatase (PGPase) require elevated levels of CO_2 for growth in the light and cannot grow when photorespiration occurs. Revertants, namely, double mutants capable of growth under air without restoration of the missing PGPase activity, might be expected to have secondary mutations that reduce or eliminate photorespiration. Nineteen revertants were selected from a culture of a PGPase-deficient mutant of Chlamydomonas reinhardtii (pgp-1-18-7F) after a second mutagenesis that involved treatment with 5-fluorodeoxyuridine and ethyl methanesulfonate. There were significant differences in the photosynthetic affinity for CO_2 among revertant cells grown under 5% CO_2. Eight revertants had five times higher photosynthetic affinity for CO_2 than that of wild type 2137 cells grown under 5% CO_2, resembling air-adapted wild-type cells, whereas four revertants had less than half the affinity for CO_2 of the wild type. In all of the revertant cells with higher affinity grown in 5% CO_2, the rates of photosynthesis under levels of CO_2 below those in air were apparently higher than that of the wild type, whereas the rates under CO_2-saturating conditions were lower than that of wild type, indicating that the efficiency of photosynthesis under air was significantly improved in these revertants. In addition, some revertants had a photosynthetic capacity and a growth rate higher than those of the wild type, without any increased photosynthetic affinity for CO_2.
- 日本植物生理学会の論文
著者
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SUZUKI Kensaku
Plant Physiology Laboratory, National Agricultural Research Center for Tohoku Region
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Suzuki Kensaku
Plant Eco-physiol Lab. Tohoku Natl. Agric. Expt. Stn.
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Suzuki Kensaku
Plant Eco-physiology Laboratory Tohoku National Agricultural Experiment Station
関連論文
- cAMP Responsive Element-like Sequences Are Detected in the Upstream Region of a Mating Gene of the Green Alga, Chlamydomonas reinhardtii
- AN EUKARYOTIC PHOSPHOGLYCOLATE PHOSPHATASE FROM CHLAMYDOMONAS
- Phosphoglycolate Phosphatase-Deficient Mutants of Chlamydomonas reinhardtii Capable of Growth under Air : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- PURIFICATION AND SOME PROPERTIES OF PHOSPHOGLYCOLATE PHOSPHATASE FROM CHLAMIDOMONAS REINHARDTII
- CHLAMYDOMONAS MUTANTS WITH LOWER ACTIVITY OF PHOTORESPIRATION
- MEASUREMENT OF THE RATE OF PHOTORESPIRATION IN Chlamydomonas MUTANTS.
- A Mutant of Chlamydomonas reinhardtii with Reduced Rate of Photorespiration
- CHARACTERISTICS OF CHLAMYDOMONAS MUTANTS WITH REDUCED RATE OF PHOTORESPIRATION (3)
- CHARACTERISTICS OF CHLAMYDOMONAS MUTANTS WITH REDUCED RATE OF PHOTORESPIRATION (2).
- CHLAMYDOMONAS MUTANTS WITH REDUCED RATE OF PHOTORESPIRATION
- PHOSPHOGLYCOLATE LEVELS IN PGP1 MUTANTS OF CHLAMYDOMONAS