Regulation of the Cytosolic Fructose-1,6-Bisphosphatase by Post-Translational Modification and Protein Level in Drought-Stressed Leaves of Sugarbeet : GENE STRUCTURE AND EXPRESSION

スポンサーリンク

概要

• 論文の詳細を見る

• Sugarbeet plants (Beta vulgaris, L.) were grown in a growth chamber and subjected to a slow-developing (in vivo) period of drought stress by withholding water. During an 8 day period, several parameters were monitored. A gradual and consistent decline was observed in the activity of the cytosolic fructose-1,6-bisphosphatase (FBPase) during the first 3-4 days of stress. During this initial period, leaf water potential had declined from -0.8 MPa, but no significant changes were observed in hexose, sucrose and starch contents, levels of FBPase protein and transcript and sucrose-phosphate synthase (SPS) transcript. Major changes in all parameters occurred during the latter stages of stress. At the end of this period, water potential was -4.3 MPa, sucrose levels increased three fold, starch content declined to less than 10% and FBPase activity was about 40% of controls. SPS activity slightly declined under substrate-saturating conditions but increased (100%) under substrate-limited conditions. SPS transcript slightly declined only in severely stressed leaves. Protein and transcript levels of FBPase also declined, but substantial amounts remained in these leaves suggesting that sugarbeet has a relatively resilient system for sucrose synthesis. During the initial stages, the decline in FBPase activity was coupled with stable protein level, suggesting post-translational modification of the protein. Later, regulation appeared to involve decline in the protein level. The data provide the first evidence for the coarse control of this enzyme in plants.

• 日本植物生理学会の論文

著者

• Harn C

  Rutgers Univ. Nj Usa

• Daie Jaleh

  Department of Crop Science, Lipman Hall, P. O. Box 231, Cook College, Rutgers University

• Harn Chee

  Department of Crop Science, Rutgers University, Cook College

• Daie Jaleh

  Department Of Botany Birge Hall University Of Wisconsin

• Daie Jaleh

  Department Of Crop Science Rutgers University Cook College

関連論文

• Growth and Sink to Source Transition in Developing Leaves of Sugarbeet
• Does Apoplastic Sucrose Induce the Ability for Sucrose Loading in Developing Leaves of Sugarbeet?
• Regulation of the Cytosolic Fructose-1,6-Bisphosphatase by Post-Translational Modification and Protein Level in Drought-Stressed Leaves of Sugarbeet : GENE STRUCTURE AND EXPRESSION
• Expression Dynamics of Genes Encoding Key Carbon Matabolism Enzymes during Sink to Source Transition of Developing Leaves
• Expression of Genes Encoding Rubisco in Sugarbeet(Beta vulgaris.L)Plants Subjected to Gradual Desiccation : ENVIRONMENTAL AND STRESS RESPONSES

スポンサーリンク