Conversion of (+)-Dihydroquercetin to 3,4-cis-Leucocyanidin by a Reductase Extracted from Cell Suspension Cultures of Cryptomeria japonica

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概要

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• A soluble, NADPH-dependent reductase catalyzing the reduction of (+)-dihydroquercetin to 3,4-cis-leucocyanidin (5,7,3',4'-tetrahydroxyflavan-3,4-cis-diol) was demonstrated in an enzyme preparation from a cell suspension culture of Japanese cedar (Cryptomeria japonica D. Don). The K_m value for (+)-dihydroquercetin was 48μM. The enzyme, which was purified 26.2-fold, could also catalyze the reduction of (+)-dihydrokaempferol to 3,4-cis-leucopelargonidin (5,7,4'-trihydroxyflavan-3,4-cis-diol). The enzyme had a pH optimum of 7 and a molecular weight of 133.000. It was inhibited by Cu^<2+> and iodoacetate, but not by p-chloromercuribenzoate. Dur-ing the growth stages of the cell suspension cultures, an increase in reductase activity proceeds an increase in procyanidin content, as might be expected.

• 日本植物生理学会の論文

著者

• MURAKAMI Hiroyuki

  Department of Physics, Rikkyo University

• Murakami Hidetoshi

  Department Of Applied Physics Hokkaido University

• Fujii Yuji

  Department of Thoracic Surgery, International Medical Center of Japan

• Murakami H

  Nhk Sci. And Technical Res. Lab. Tokyo Jpn

• Murakami H

  Department Of Applied Chemistry Graduate School Of Engineering Tokyo University Of Agriculture And T

• Fujii Yuji

  Department Of Biology Faculty Of Science Kumamoto University

• ISHIKURA Nariyuki

  Department of Biological Science, Faculty of Science, Kumamoto University

• Murakami H

  Department Of Applied Physics Hokkaido University

• Ishikura Nariyuki

  Department Of Biological Science Faculty Of Science Kumamoto University

• Murakami Hiroyuki

  Department Of Aerospace Engineering Tokyo Metropolitan Institute Of Technology

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