Hydrogen Peroxide-Dependent Oxidation of Flavonoids and Hlydroxycinnamic Acid Derivatives in Epidermal and Guard Cells of Tradescantia virginiana L.
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概要
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Luteolin, kaempferol, quercetin, caffeic acid and ferulic acid were identified in acid-hydro-lyzed epidermal strips of Tradescantia virginiana using HPLC and spectrophotometry. The amount of flavonoids was much smaller than that of cinnamic acid derivatives. More than 80% of the flavonoids were found in methanol extracts of epidermal strips. Caffeic acid was found in both methanol extracts and the residues in nearly equal amounts, while more than 80% of the ferulic acid was found in the residues after methanol extraction. These data suggest that most of the ferulic acid and part of the caffeic acid bind to macromolecules as esters in the cell wall and that flavonoids are localized mainly in the cytoplasm. The localization of esters of hydroxycinnamic acids in cell walls was ascertained by fluorometric analysis. These phenolic compounds were oxidized by H_2O_2 (0.025-1 mM) in epidermal and guard cells and the oxidation was inhibited by KCN and NaN3: Iuteolin glycosides were less sensitive to H_2O_2 than quercetin and kaempferol glycosides in flavonoids. Ferulic acid esters were more sensitive to H_2O_2 than caffeic acid esters in hydroxycinnamic acid derivatives. On the basis of these data, the physiological significance of the oxidation of phenolic compounds by H_2O_2 is discussed.
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