Purification of Protein Body-I of Rice Seed and its Polypeptide Composition

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概要

• 論文の詳細を見る

• Protein body type one (PB-I) was isolated and purified from developing rice grain by a combination of sucrose density gradient centrifugation and treatment with pepsin. SDS-PAGE analysis showed that isolated PB-I contains several polypeptide groups, the largest having an apparent molecular size of 13 kDa and two smaller ones of 10 kDa and 16 kDa. The 13-kDa group was found to be composed of two polypeptides of slightly different molecular sizes, 13a (larger component) and 13b (smaller component). Most of the 13a and 13b polypeptides were shown to be largely prolamins, although there were also some salt- and alcohol-insoluble polypeptides with an apparent molecular size of 13 kDa. It was concluded that PB-I is the accumulation site of rice prolamin. It was further estimated that the protein amount in PB-I accounted for about 20% of the total protein of rice endosperm.

• 日本植物生理学会の論文

著者

• SATOH Hikaru

  Institute of Genetic Resources, Faculty of Agriculture, Kyushu University

• Iwata N

  Istec Tokyo

• Iwata N

  Department Of Physics Faculty Of Science Yamaguchi University

• Iwata N

  Kyushu Univ. Fukuoka

• Iwata N

  Kyushu Univ. Fukuoka Jpn

• Iwata Nobuo

  Department Of Physics Yamaguchi University

• Iwata Nobuo

  Plant Breeding Laboratory, Faculty of Agriculture,Kyushu University

• Satoh H

  Toho Univ. Funabashi Jpn

• Ogawa Masahiro

  Research Institute for Food Science, Kyoto University

• Kumamaru Toshihiro

  Plant Breading Laboratory, Faculty of Agriculture, Kyushu University

• Satoh Hikaru

  Experimental Farm, Kyushu University

• Omura Takeshi

  Plant Breading Laboratory, Faculty of Agriculture, Kyushu University

• Kasai Zenzaburo

  Plant Nutrition Laboratory, Faculty of Agriculture, Kinki University

• Tanaka Kunisuke

  Department of Biochemistry, Faculty of Agriculture, Kyoto Prefectural University

• Kumamaru Toshihiro

  Faculty Of Agriculture Kyushu University

• Kumamaru Toshihiro

  Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Iwata Nobuo

  Plant Breading Laboratory Faculty Of Agriculture Kyushu University

• Kasai Zenzaburo

  Plant Nutrition Laboratory Faculty Of Agriculture Kinki University

• Ogawa Masahiro

  Faculty Of Human Life Science Yamaguchi Prefectural Univeresity

• Omura Takeshi

  Plant Breading Laboratory Faculty Of Agriculture Kyushu University

• Tanaka Kunisuke

  Department Of Biochemistry Faculty Of Agriculture Kyoto Prefectural University

• Tanaka Kunisuke

  Department Of Agricultural Chemistry Kyoto Prefectural University

• Satoh Hikaru

  Experimental Farm Kyushu University

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