Purification and Characterization of an Aminopeptidase, LPAase 2, from Euonymus Leaves
スポンサーリンク
概要
- 論文の詳細を見る
An aminopeptidase, LPAase 2, from the leaves of Euonymus alatus f. ciliato-dentatus was purified about 240-fold by a combination of DEAE-cellulose and Sephadex G-100 column chromatographies. The molecular weight of LPAase 2 was estimated to be about 62,000, and the optimum pH for the hydrolytic activity against leucine-p-nitroanilide (LPA) was 7.6. LPAase 2 hydrolyzed LPA, leucine-β-naphthylamide (leucine-NA), phenylalanine-NA and tyrosine-NA. It was inhibited strongly by p-chloromercuribenzoate (PCMB), iodoacetic acid and heavy metal ions, but was not affected by thiol compounds and metal-chelating reagents. Therefore, a sulfhydryl group could be involved in the active site of LPAase 2. None of the metal ions tested promoted LPAase 2 activity. The properties of LPAase 2 were compared with those of aminopeptidases reported for other plants.
- 日本植物生理学会の論文
著者
-
Tazaki Kiyoshi
Department Of Biology Faculty Of Science Kumamoto University
-
Ishikura Nariyuki
Department Of Biological Science Faculty Of Science Kumamoto University
関連論文
- A Stable Reddish Purple Anthocyanin in the Leaf of Gynura aurantiaca cv. 'Purple Passion'
- Conversion of (+)-Dihydroquercetin to 3,4-cis-Leucocyanidin by a Reductase Extracted from Cell Suspension Cultures of Cryptomeria japonica
- Anthocyanins in the Black Purple Fruits of Vitis Flexuosa Thunb.
- Detection and Characterization of an S - Adenosyl - L - methionine : Flavonoid 7 - 0 - Methyltransferase from Leaves of Prunus × yedoensis Matsum. with Special Reference to Prunetrin Formation
- Effects of Glyphosate on the Shikimate Pathway and Regulation of Phenylalanine Ammonia-lyase in Cryptomeria and Perilla Cell Suspension Cultues
- Partial Purification and Some Properties of Flavonol 3-O-Glycosyltransferases from Seedings of Vigna mungo, with Special Reference to the Formation of Kaempferol 3-O-Galactoside and 3-O-Glucoside
- Notes on the Anthocyanidin Types and Metals in Blue Flowers and Fruits
- The Flavonoid Chemistry of the Genus Trillium I. Flavonol Glycosides in the Leaves of Trillium tschonoskii Maxim. and Their
- Effects of Glyphosate on Caffeic Acid Metabolism in Perilla Cell Suspension Cultures
- Purification and Characterization of an Aminopeptidase, LPAase 2, from Euonymus Leaves
- Isolation of a New Flavonol Glycoside and its Effects on the Blue Color of Seed Coats of Ophiopogon jaburan
- Alicyclic acid metabolism in plants V. Biosynthesis of alicyclic acids from ^C-labeled glucose and erythrose in some plant tissues
- Detection and Characterization of UDP-Glucose:Flavonoid O-Glucosyltransferases in the Leaves of Prunus x yedoensis Matsum.
- Paper Chromatographic Survey of Athocyanin in Turip-Flowers, II
- Studies on the flower color of morning-glory I. : Anthocyanins and some concomitant phenolics in the blue petals of Ipomoea ruburo-caerulea Hook, 'Heavenly Blue'
- Preliminary Report on the Anthocyanins in the Flowers of Veronica persica
- Complex (Aroylated) Anthocyanins Found in the Flowers of Hyacinth
- On the Anthocyanin Containing Branched Trisaccharide Found in Fruit Skin of Kadsura japonica
- Anthocyanins in the Flowers of Japanese Garden Iris Belonging to 'Higo' Line
- Paper chromatographic survey of anthocyanins in four plants belonging to the family Boraginaceae
- Biosynthesis of alicyclic acids from ^C-labeled glucose and erythrose by isolated cells from Vigna angularis leaves
- Anthocyanins and Some Factors Involving in the Flower Colors of Morning-glory Belonging to 'Higo' Line
- The Changes in Anthocyanin and Chlorophyll Content During the Autumnal Reddening of Leaves
- Purification and Characterization of an Aminopeptidase, the Enzyme-Hydrolyzing Alanine-p-nitroanilide (APAase), from Euonymus Leaves
- Multiple Forms of Aminopeptidase in Euonymus Leaves
- Light absorption patterns of anthocyanin-containing cells
- Anthocyanins and Flavones in Leaves and Seeds of Perilla Plant
- Flavonol Glycosides in the Flowers of Hibiscus mutabilis f. oversicolor