CHANGES OF FREE AMINO ACID COMPOSITION IN TOBACCO LEAVES DURING THE PROCESS OF "FLUE-CURING"
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概要
- 論文の詳細を見る
Although considerable attention has been paid to the change of amino acids in tobacco leaves during the process of "curing", comparatively little is known of the quantitative changes of individual amino acids, and nothing is known of the behaviors of such amino acids as proline, hydroxyproline and γ-aminobutyric acid during the process. The present study deals with the changes in content of amino acids and related compounds in tobacco leaves during the process of "flue-curing". In this method, which is now usually in practice in Japan, the tobacco leaves harvested from the farm are placed in a drying room ("burn") under artificial control of temprature and humidity. The temperature is adjusted at first at 37° and gradually raised up to 72° in about 90 hours which is required for the completion of the process. Materials used were well matured middle leaves taken from the tobacco plants cultivated in the farm of Hatano Tobacco Experiment Station in 1959. At various stages of curing, aliquots of leaves were taken out and quickly extracted with hot 80% ethanol. Amino acids were determined by the method of MOORE and STEIN (1, 2). The contents of asparagine and glutamine were determined as the differences in content of aspartic and glutamic acids, respectively, before and after the hydrolysis of the alcohol extract. The results obtained are summarized in Table I and II. Content of free amino nitrogen was found to increase almost 10 times during the process. Considerable increases were observed in proline, hydroxyproline, phenylalanine, γ-aminobutyric acid, α-alanine, asparagine, glutamine, ammonia and some unknown fractions. In lesser amounts were contained glutamic acid, aspartic acid, serine, cystine, valine, methionine and tyrosine, whose contents also changed during the curing.
- 日本植物生理学会の論文
著者
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Yoshida Daisuke
Hatano Tobacco Experiment Station Japan Monopoly Corporation
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Yoshida Daisuke
Hatano Tabacco Experiment Station The Japan Monopoly Corporation
関連論文
- CHANGES OF FREE AMINO ACID COMPOSITION IN TOBACCO LEAVES DURING THE PROCESS OF "FLUE-CURING"
- DEGRADATION AND TRANSLOCATION OF ^N-LABELED NICOTINE INJECTED INTO INTACT TOBACCO LEAVES
- Effect of agmatine on putrescine formation in tobacco plants
- AGMATINE AND N-CARBAMYLPUTRESCINE AS INTERMEDIATES IN THE FORMATION OF NICOTINE BY TOTBACCO PLANTS
- STUDIES ON INTERRELATIONSHIP OF VARIOUS IONS IN ABSORPTION BY TOBACCO PLANTS : (PART 2) RELATION OF NITROGEN FORMS AND PHOSPHORUS LEVELS IN CULTURE SOLUTION
- EFFECTS OF FORMS OF NITROGEN SUPPLIED ON THE DISTRIBUTION OF NUTRIENTS IN THE TOBACCO PLANT
- DETERMINATION OF BIURET IN UREA BY IONEXCHANGE RESINS
- RELATION BETWEEN THE BEHAVIOUR OF NITROGEN AND THE NICOTINE SYNTHESIS IN TOBACCO PLANT
- STUDIES ON INTER-RELATIONSHIP OF VARIOUS IONS IN ABSORPTION BY TOBACCO PLANTS III. : Relation of Magnesium and Phosphorus Levels in Culture Solution
- INFLUENCES OF NITROGEN NUTRITION ON NICOTINE SYNTHESIS IN TOBACCO PLANT
- TRANSLOCATION OF SERINE FROM LEAVES TO ROOTS IN TOBACCO PLANTS
- FRACTIONATION OF FOLIAR ABSORBED PHOSPHORUS BY PAPER ELECTRO-PHORESIS
- EFFECTS OF NUTRIENT DEFICIENCIES ON THE BIOSYNTHESIS OF NICOTINE IN TOBACCO PLANTS
- EFFECT OF NITROGEN NUTRITION ON THE FREE AMINO ACID COMPOSITION IN TOBACCO PLANT
- STUDIES ON INTERRELATIONSHIP OF VARIOUS IONS IN ABSORPTION BY TOBACCO PLANTS : (PART 1) RELATION OF NITROGEN FORMS AND CATION LEVELS IN CULTURE SOLUTION
- STUDIES ON INTER-RELATIONSHIP OF VARIOUS IONS IN ABSORPTION BY TOBACCO PLANTS IV. : Relation of Magnesium, Potassium and Calcium in Culture Solution