Control of phosphatase release from cultured tobacco cells

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概要

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• The activity of cultured tobacco XD-6 cells to release phosphatase into the medium was enhanced after a time lag of 2 to 3 days following a marked increase in intracellular level of the enzyme during Pi-omitted culture. The enhanced release of phosphatase had a Q_<10> value of about 2.3, and was suppressed by 2,4-dinitrophenol and azide. Cycloheximide did not inhibit the enzyme release, but Pi caused a rapid and drastic decrease in the rate of release. The release is suppressed even by 1 μM Pi. These results indicate that the rate of enzyme release is not directly limited by synthesis of the enzyme, but limited by the transport, which may be suppressed by Pi, of the enzyme to the outside of the cell membrane.

• 日本植物生理学会の論文

著者

• Ueki Katsuji

  Department Of Botany Faculty Of Science University Of Tokyo

関連論文

• Sample Preparation for Methanogenic Activities of Heterogeneous Flooded Rice Soils
• Chromatographic separation of extracellular acid phosphatase of tobacco cells cultured under Pi-supplied and omitted conditions
• Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells
• Control of phosphatase release from cultured tobacco cells

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