Phytochemical studies on tobacco alkaloids XIV. The occurrence and properties of putrescine N-methyltransferase in tobacco roots
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概要
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Putrescine N-methyltransferase, a new enzyme catalyzing the formation of N-methylputrescine from putrescine and S-adenosyl-L-methionine was found in roots of tobacco plants. The enzyme was purified 30-fold from crude extracts of tobacco roots. N-Methylputrescine was identified as the reaction product by comparison with the authentic compound. The enzyme had a pH optimum between pH 8 and 9, and a molecular weight of about 60,000, as determined by gel filtration. Km values for putrescine and S-adenosyl-L-methionine were 4.0×10^<-4> M and 1.1×10^<-4> M, respectively. Enzyme activity was inhibited by p-chloromercuribenzoate and Ag^+. No cofactors were required. Of the various substrates tested, only putrescine served as a methyl acceptor. The enzyme was localized exclusively in the roots and its activity was greatly enhanced by decapitation. The presence of putrescine N-methyltransferase in tobacco roots strongly suggests that N-methylputrescine participates as an intermediate in nicotine biosynthesis.
- 日本植物生理学会の論文
著者
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Tanabe Yoko
The Central Research Institute Japan Monopoly Corporation
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Noguchi Masao
The Central Research Institute Japan Monopoly Corporation
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Noguchi Masao
The Central Research Institute Japan Monopoly Corp.
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TAMAKI EINOSUKE
The Central Research Institute, Japan Monopoly Corporation, Nishishinagawa
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Mizusaki Shigenobu
The Central Research Institute, Japan Monopoly Corporation
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Mizusaki Shigenobu
The Central Research Institute Japan Monopoly Corporation
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Tamaki Einosuke
The Central Research Institute Japan Monopoly Corporation
関連論文
- Metabolism of glutamate and γ-aminobutyrate in cultured tobacco cells
- Studies on nitrogen metabolism in tobacco plants IX. Effect of various compounds on proline biosynthesis in the green leaves
- Changes in the activities of ornithine decarboxylase, putrescine N-methyltransferase and N-methylputrescine oxidase in tobacco roots in relation to nicotine biosynthesis
- Phytochemical studies on tobacco alkaloids XIV. The occurrence and properties of putrescine N-methyltransferase in tobacco roots