新規抗癌剤1-(β-Chloroethyl)-2-chloromethylpiperidine Hydrobromideの人由来継代培養細胞に対する致死効果

概要

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Based on the previous observation that various rat ascites hepatoma cell lines showed different sensitivity to the new antitumor agent, bis (chloroalkyl) piperidine, and the possibility that this difference reflected the repair ability of the cells for damaged DNA, which might also be considered in the case of human cancer cells, the lethal action of 1-(β-chloroethyl)-2-chloromethylpiperidine hydrobromide (CAP-1) on colony-forming ability of the cells was examined using various human tissue cultured cell lines derived from tumor and normal tissues. The results of these experiments showed different effective concentrations for CAP-1 sensitivity of the cell lines at 37% (e^<-1>) viability. The level of CAP-1 sensitivity of the cell lines agreed with their sensitivity to ultraviolet ray (UV) and nitrogen mustard N-oxide. It is well known that the level of UV-sensitivity reflects repair-ability of the cells, and DNA damage produced by alkylating agents may be repaired by the UV-repair (excision repair) mechanism, or at least by a mechanism sharing some of the same steps. Therefore, from these results and evidences, it may be concluded that the sensitivity of human cultured cell lines to chemicals is closely related to their repair ability.
公益社団法人日本薬学会の論文
1978-11-25