チトクロムc.ヘムオクタペプチド(CHP)の生理活性その3脂肪酸過酸化物の生成抑止と分解促進に対するCHPの影響

概要

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An octapeptide fragment containing the heme moiety (CHP), which was prepared from Candida krusei cytochrome c, showed a protective effect against the denaturation of cells and organelles due to oxygen deficiency in various types of experimental hypoxia. In vivo and in vitro experiments were carried out to investigate the effect of CHP on the lipid peroxide as a result of the oxidation of unsaturated fatty acids. Lipoxidase-catalyzed oxidation was more effectively blocked by the addition of CHP solution (2×10^<-6>M) than of other antioxidants such as vitamin E and α-naphthol (1.7×10^<-5>M). Linolenic acid peroxide was decomposed in the presence of CHP, as well as other heme containing molecules, although vitamin E and α-naphthol were not effective. The increase of lipid peroxide formation in liver tissues in rabbits fed on a vitamin Edeficient diet was proved by the colorimetric thiobarbituric acid method (TBA). The TBA values of CHP-treated group (0.5 mg/kg/day, 4 weeks) were significantly lower than that of saline control (p<0.05). Liver homogenates of rats in hemic hypoxia also formed much lipid peroxide as measured by the TBA method, and the degree of increase in CHP-treated group (1 mg/kg) was somewhat lower. These results suggested that CHP may serve as a protective agent against cellular damage in hypoxia, when its native cytochrome c or itself is properly administered.
公益社団法人日本薬学会の論文
1975-06-25