DPPCリポソームの糖による構造保護メカニズム
スポンサーリンク
概要
- 論文の詳細を見る
Molecular mechanism of structure-protection of dipalmitoylphosphatidylcholine (DPPC) liposomes by sucrose adsorption on/in the liposome surface was studied by performing fluorescence decay, quartz-crystal microbalance (QCM), and infrared reflection-absorption (IRRA) spectroscopic measurements. Thermally hydrated DPPC monolayers were newly introduced in place of the conventional DPPC liposomes, since the hydrated monolayers were expected to have similar surface property to that of liposomes. Studies of the fluidity and permeability of membrane in the monolayers and liposomes suggested that the thermally hydrated DPPC monolayers could be used as a model membrane in place of DPPC liposomes. With the hydrated DPPC monolayers, the adsorption rate of sucrose molecules in the DPPC monolayer was investigated by a combined technique of QCM and IRRA by the use of Langmuir-Blodgett (LB) technique. It was found that the incorporation rate of sucrose into the monolayer in the liquid-expanded state was proportional to the bulk concentration of sucrose in the Langmuir trough. The linear relationship depicts the incorporation mechanism that sucrose molecules were deeply incorporated in the DPPC molecules, and the layer is formed as a single mingled layer. This molecular scheme readily supports the conventional hypotheses based upon water-replacement and vitrification mechanisms. The IRRA spectra were further studied using newly found property of principal component analysis (PCA). PCA was found to have great potential to detect minute bands hidden in dominantly large bands, after theoretical speculation and simulations. The new analytical technique was employed to search for an unknown minute spectrum in the collection of raw spectra. The calculated abstract spectra yielded by PCA strongly suggested that there remained minute quantity of water in the dried LB films. The water molecules were also found to bind to PO_2 group in the head group moiety of DPPC. The mass change of adsorbed and incorporated sucrose molecules in the DPPC monolayer were readily distinguished by PCA without any probe molecule, and they were followed semi-quantitatively by calculation of scores.
- 社団法人日本薬学会の論文
- 2000-01-01
著者
関連論文
- 赤外分光測定法-基礎と最新手法第I部 基礎編 : 第4回6. 赤外分光におけるケモメトリックス
- 仮想光計測という概念が拓く新しい薄膜構造解析 : 赤外MAIR分光法の原理と生体薄膜の構造解析
- 記録の価値 : 分析化学の楽しみ
- 多角入射分解赤外分光法の原理と実際
- 赤外分光法を用いた界面のはかりかた
- 計量化学が拓く新しい界面の光計測(計量生物工学-ポストIT時代の生物工学)
- 赤外MAIR分光法による階層構造化超分子フィルムの構造解析
- ロイシンファスナーでβ-シートを組織化した超分子単分子膜
- 超分子の階層構造化による新しい超分子ポリマーの創出 (特集 非共有結合性高分子(Part2))
- 多変量スペクトル解析による微量化学種検知
- DPPCリポソームの糖による構造保護メカニズム
- LC/MSなどにおけるスペクトルの高SN比解析法
- 赤外反射分光法による有機吸着薄膜の解析
- Open-path FTIRによる大気中のガス分析
- 赤外外部反射分光法による表面分析 -非金属基板上の有機超薄膜の分子配向解析-
- Effect of Substrates on the Infrared External Reflection Spectra of Langmuir-Blodgett Films
- 機能性高分子の細胞内導入のための新しい基礎研究
- たわみ振動板を用いた小型超音波ポンプ(強力超音波)
- 赤外分光測定法-基礎と最新手法第II部 各種測定法 : 第5回7A. 正反射測定法
- 赤外分光測定法-基礎と最新手法第II部 各種測定法 : 第5回7B. 薄膜・界面の外部反射測定法
- 多変量解析が結ぶ分光学と分析化学 (特集/分光・分析研究と応用)
- 日本分析化学会第60年会