トリプトファン代謝産物の微量分析とマウス遅延性アレルギー抑制因子の研究

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概要

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• We developed methods for the fluorometric assay of 3-hydroxykynurenine and 3-hydroxy-anthranilic acid, which are suspected as carcinogens in bladder cancer. It was shown that the urinary excretion of 3-hydroxyanthranilic acid increased in patients with bladder cancer. We also developed methods for the fluorometric assay of glucuronide and sulfate of 3-hydroxyanthranilic acid and showed that the excretion of these conjugated forms was minor in humans. The distribution of 3-hydroxykynurenine was studied and data obtained suggested that it has an affinity for the pancreas. We then developed methods for determination of the related compounds of tryptophan. Fluorescence reaction with UV radiation was applied to the determinations of kynurenic acid, kynurenine, quinolinic acid, nicotinic acid, nicotinamide, N^1-methyl-nicotinamide, isatin, xanthrenic acid, and melatonin in the serum or urine. Furthermore, the fluorescence reaction with UV radiation was applied to some drugs, e.g., indomethacin, isoniazid, naldixic acid, nicorandil, and disodium cromoglycate. The relationship was investigated between the tumor promoter, 12-tetradecanoyl-phobol-13-acetate (TPA), and delayed hypersensitivity in mice. The foot pad reaction (FPR) in mice was suppressed by the application of TPA following the application of 7, 12-dimethylbenz [α]-anthracene (DMBA), a tumor initiator, in BALB/c mice, while the FPR was suppressed by the application of TPA alone in C3H/He mice. CD8^+ and CD4^+ T cells, which suppress the FPR, were induced in BALB/c and C3H/He mice, respectively. These T cells produced soluble factors that inhibited the FPR in mice.

• 公益社団法人日本薬学会の論文
• 2002-07-01

著者

• 渡辺 光夫

  帝京大学薬学部薬品分析学教室

• 渡辺 光夫

  帝京大学薬学部

関連論文

• Enzymatic Formation of Xanthurenic Acid 8-Methyl Ether, an Endogenous Carcinogen, in Animal Tissues
• Distribution of Tryptophan Metabolites in the Organs of Mice
• 高速液体クロマトグラフィーによる生体試料中の3-ヒドロキシアントラニル酸の蛍光定量(超微量分析のための前処理と予備濃縮)
• Suppression of Delayed-Type Hypersensitivity in Mice by 12-Tetradecanoylphorbol-13-acetate
• 酢酸亜鉛(II)試薬を用いる尿中キヌレン酸の蛍光分析
• Simultaneous Fluorometric and Colorimetric Detection of Carbohydrates on Silica Gel Plates using o-Aminobenzenesulfonic Acid
• トリプトファン代謝産物の微量分析とマウス遅延性アレルギー抑制因子の研究
• Tryptophan代謝産物の微量分析の研究を中心として
• 国際癌学会
• 分析化学実習書

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