副腎摘出ラットにおける鉛誘導亜鉛チオネイン

概要

論文の詳細を見る
At 18 h after the sham operation or adrenalectomy in male Wistar rats, a low molecular weight (about 10000 daltons) zinc-thionein was observed in the hepatic 105000g soluble fraction on a Sephadex G-75 gel filtration column. The zinc-thionein level returned nearly to the control level 5 d after both operations. In the sham-operated or adrenalectomized rats at 5 d after the operation, a single injection of lead acetate or cadmium chloride at 18 h prior to sacrifice clearly induced the zinc-thionein. On the other hand, zinc content in the serum decreased markedly at 18 h after adrenalectomy. A single administration of lead and cadmium immediately or 4 d after the sham operation or adrenalectomy significantly reduced zinc content in the serum. Total zinc content in the liver was increased by the administration of metals when compared to that of the control. However, the distribution ratio of zinc content in subcellular fractions, namely 600 g ppt, mitochondria, microsome, and 105000g soluble fraction, was similar to that in the control and metal treated rats. Our results in adrenalectomized rats suggest that the induction mechanism of lead-indued zincthionein is distinct from that of adrenal related induced metallothionein.
公益社団法人日本薬学会の論文
1984-12-31