ペルオキシダーゼ, 過酸化水素および4-アミノアンチピリンを用いる水中フェノール類の吸光光度定量法

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In order to establish a simple method for the determination of phenols in river water and industrial waste water, conditions were examined for the reaction used for the determination of hydrogen peroxide ; the reaction using 4-aminoantipyrine, phenol, hydrogen peroxide, and peroxidase, and the following method was established. The sample water (200ml) is distilled, 50 ml of its distillate is placed in a 50 ml test tube with a stopper, and 2 ml of 1 M phosphate buffer (pH 7.25) containing 5 mg of 4-aminoantipyrine and 0.5 mg of an enzyme (horseradish peroxidase), and 1 ml of 0.3% hydrogen peroxide solution are added, and the mixture is incubated at 37° for 15 min in a water bath. The reaction mixture is cooled to 5° and absorbance of this colored solution is read at 510 nm. Recovery of phenol added to distilled water by this method was 98.9-99.7% and its coefficient of variation was 0.61-0.84%. Coloration rate of phenols by this method was compared with the rate by the ferricyanide method, using 52 kinds of phenolic compounds, and the total of coloring rate by the present method was higher than that by the ferricyanide method. Correlation between the result of the present method and that of the ferricyanide method was examined with river water and industrial waste water collected in Wakayama city. Calculation of the linear regression line (y=1.119x+6.364) and correlation coefficient (r=0.943) indicated correlation between these methods.
公益社団法人日本薬学会の論文
1978-08-31

ペルオキシダーゼ, 過酸化水素および4-アミノアンチピリンを用いる水中フェノール類の吸光光度定量法

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