Analysis of DMA Adducts after Exposure to 1, 4-Dichlorobenzene by ^<32>PPostlabeling Technique
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概要
- 論文の詳細を見る
To evaluate the genetic toxicity of 1, 4-dichlorobenzene (p-DCB), DNA adducts of p-DCB were analyzed in in vitro and in in vivo. In the in vitro study, calf thymus DNA (400μg/ml) was mixed with p-DCB (100μM), liver microsome (1 mg protein/ml) and an NADPH-regenerating system. After incubation at 37℃ for 1 hr, DNA was isolated and purified for DNA adduct analysis. There were no DNA adducts detected when p-DCB was metabolized by rat, mouse or human liver microsomes. In the in vivo study, Fischer 344 rats were given different kinds of cytochrome P450 (CYP) inducers and then injected with p-DCB. Twenty-four hr after injection, livers were collected from the rats for DNA adducts analysis. We did not find any p-DCB DNA adducts in rat livers pre-treated with ethanol, phenobarbital or 3-methylcholanthrene, respectively. In conclusion, no p-DCB DNA adducts were found either the in vitro or in vivo studies. In this study, we demonstrated that p-DCB is not genotoxic.
- 公益社団法人日本薬学会の論文
- 2001-02-01
著者
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MIYAZAWA HIROSHI
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
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GOTO Sumio
Department of Orthopaedic Surgery, Chiba University Graduate School of Medicine
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GOTO Sumio
Azabu University
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MIYAZAWA Hiroshi
NTT Electronics Corporation
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FUKUHARA MORIO
Department of Pharmaceutical Sciences, National Institute of Public Health
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Goto S
College Of Environmental Health Azabu University
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Goto S
Reseacrh Center For Material Cycles And Waste Management National Institute For Environmental Studie
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Goto Sumio
Department Of Orthopaedic Surgery Chiba University Graduate School Of Medicine
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Fukuhara Morio
Department Of Pharmaceutical Sciences
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Miyazawa H
Ntt Electronics Corporation
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Madhusree Bathini
Department of Pharmaceutical Sciences, National Institute of Pubilc Health
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Tian Hailin
Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University
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Tohkin Masahiro
Department of Pharmaceutical Sciences, National Institute of Public Health
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Tian Hailin
Department Of Physiological Sciences College Of Veterinary Medicine Oklahoma State University
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Fukuhara Morio
Department Of Pharmaceutical Sciences National Institute Of Public Health
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Tohkin M
Department Of Pharmaceutical Sciences National Institute Of Public Health
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Madhusree B
日本学術振興会
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Madhusree Bathini
Department Of Chemistry S.v.u. Engineering College S.v. University
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Goto Sumio
Department Of Community Environmental Sciences National Instite Of Public Health
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Miyazawa Hiroshi
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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Tohkin Masahiro
Department Of Pharmaceutical Sciences National Institute Of Public Health
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