A Visual Membrane Immunoassay for the Detection of Methamphetamine Using an Enzyme-Labeled Tracer Derived from Methamphetamine and Amphetamine
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概要
- 論文の詳細を見る
A visual membrane enzyme immunoassay is described for the measurement of methamphetamine in urine. To increase assay sensitivity, tracers with chemically similar structures were cross-checked with the antibodies to determine their influence on the antibody binding. Tracers of horseradish peroxidase-labeled methamphetamine (MA-HRP) and amphetamine (A-HRP) derivatives were prepared for this purpose. Significant differences in antibody specificity were found between the two tracers. Based on the results of this study, a pair of an antibody and a tracer was selected and a membrane enzyme immunoassay (EIA) was developed utilizing the competitive binding between methamphetamine and the drug-HRP tracer. UltraBind membrane (0.45μm) was used as the solid matrix to which the antibody was attached. Using diaminobenzidine substrate with Co^<2+> ion, a stable grey color appeared on the surface of membrane for MA-negative urine samples. No color appeared for MA-positive urine with a cut-off level of 0.8 ppm.
- 公益社団法人日本薬学会の論文
- 1994-07-15
著者
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Nam K
Genetic Engineering Research Institute Korea Institute Of Science And Technology
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Choi M
Bioanalysis & Biotransformation Research Center Korea Institute Of Science & Technology
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Choi Myung
Doping Control Center Korea Institute Of Science And Technology
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PARK Jongsei
Doping Control Center, Korea Institute of Science and Technology
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Park Jisoo
R & D Center Daewoong Pharmaceutical Co. Ltd.
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Park Jongsei
Doping Control Center Korea Institute Of Science And Technology
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Choi Jeongeun
Doping Control Center Korea Institute Of Science And Technology
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GOROVITS Boris
(Present address)A. N. Bach Institute of Biochemistry, Russian Academy of Sciences
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SONG EunYoung
Genetic Engineering Research Institute, Korea Institute of Science and Technology
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NAM Kyung
Genetic Engineering Research Institute, Korea Institute of Science and Technology
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Song Eunyoung
Genetic Engineering Research Institute Korea Institute Of Science And Technology
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Gorovits Boris
(present Address)a. N. Bach Institute Of Biochemistry Russian Academy Of Sciences
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Choi J
Doping Control Center Korea Institute Of Science And Technology
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