Two Molecular Species of Oxytocinase (L-Cystine Aminopeptidase) in Human Placenta : Purification and Characterization
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概要
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Two different forms of oxytocinase (L-cystine aminopeptidase, CAP; EC 3.4.11.3) were purified from the 9000 g and 105000 g precipitate fractions of human placenta homogenate by sequential chromatography on columns of hydroxyapatite, DE-32,nickel ion affinity, and Sephadex G-200. One species (CAP-I) purified from the mitochondrial/lysosomal fraction migrated on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis with an apparent molecular mass of 61 kDa; the other (CAP-II) from the microsomal fraction was composed of two subunits with molecular masses of 56 and 40 kDa. The molecular masses of CAP-I and CAP-II estimated by gel filtration were 64 and 97 kDa, respectively. The specific activities of the two species for S-benzyl-L-cysteine p-nitroanilide increased by 357- (for CAP-I) and 139-fold (for CAP-II) compared with the starting preparations. The optimal pH values toward the artificial substrate were approx. 7.4-8.0 for CAP-I and 6.8-8.0 for CAP-II. The K_m and V_<max> values toward oxytocin were 5.6 μM and 23.4 μmol/h/mg protein for CAP-I, and 38μM and 15.6 μmol/h/mg protein for CAP-II. Both enzymes were inhibited by the metal-chelating agents, EDTA and o-phenanthroline, whereas they were specifically activated by addition of Co^<2+> : CAP-I was more sensitive to these reagents than CAP-II. L-Methionine strongly inhibited CAP-I, while CAP-II activity was only slightly affected. CAP-II was more sensitive to amastatin than CAP-I. Thus, the two enzymes are quite distinct in their molecular nature and biochemical properties. They may play a regulatory role in the metabolism of oxytocin and other biologically active peptides in intact placenta.
- 公益社団法人日本薬学会の論文
- 1997-01-15
著者
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Kawarabayashi Tatsuhiko
Department of Obstetrics and Gynecology, Faculty of Medicine, Fukuoka University
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SHIMENO Hiroshi
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Fukuoka University
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SOEDA Shinji
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Fukuoka University
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Soeda Shinji
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Fukuoka University
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Nagamatsu A
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Fukuoka University
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ITOH Chie
Department of Pharmacology, Osaka University of Pharmaceutical Sciences
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Shimeno Hiroshi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Fukuoka University
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Watanabe Maho
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Fukuoka University
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Kawarabayashi Tatsuhiko
Department Of Obstetrics And Gymecology Fukuoka University School Of Medicine
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Itoh Chie
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Fukuoka University
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NAGAMATSU Atsuo
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Fukuoka University
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