CHANGES IN GLYCOSAMINOGLYCAN SYNTHESIS DURING THE DIFFERENTIATION OF HL-60 CELLS INDUCED BY 12-O-TETRADECANOYLPHORBOL-13-ACETATE
スポンサーリンク
概要
- 論文の詳細を見る
The changes in glycosaminoglycan (GAG) synthesis during the differentiation of HL-60 cells to macrophage-like cells by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) were studied by using ^<35>S-sulfate or ^3H-glucosamine as a tracer. The incorporation of ^<35>S-sulfate into GAGs of HL-60 cells treated with TPA (TPA-treated HL-60 cells) decreased by 30% compared with untreated HL-60 cells (HL-60 cells). The profiles of the Sepharose CL-6B chromatography showed that the molecular weight size of proteoglycans (PGs) in the medium fraction of TPA-treated HL-60 cells was larger than that of HL-60 cells although the PGs in the cell fractions of both HL-60 and TPA-treated HL-60 cells were of the same molecular weight size. On the other hand, the molecular weight sizes of GAGs in both the medium and the cell fractions of TPA-treated HL-60 cells were larger than those of HL-60 cells. In order to examine the synthesis of GAG chains, HL-60 and TPA-treated HL-60 cells were incubated with ^<35>S-sulfate in the presence of 4-methylumbelliferyl-β-D-xyloside, an exogenous initiator. The incorporation of ^<35>S-sulfate into total GAGs of the TPA-treated HL-60 cells exposed to the β-D-xyloside increased 3-fold over that of HL-60 cells. HL-60 cells synthesized core protein-initiated GAGs and β-D-xyloside-initiated GAGs but TPA-treated HL-60 cells synthesized β-D-xyloside-initiated GAGs only. β-D-Xyloside-initiated GAGs synthesized by both cells were obtained in the same fraction by Sepharose CL-6B chromatography. In addition, alterations in the chain length and synthetic activity of GAGs gradually occurred with the morphological changes and came to constant values 36 h after treatment with TPA. These results suggest a very close relationship with the monocytic differentiation of HL-60 cells. However, it is not possible at present to decide whether such changes in PG synthesis result from chain length only or induction of different PGs.
- 公益社団法人日本薬学会の論文
著者
-
Mori Y
Department Of Biochemistry Tokyo College Of Pharmacy
-
Mori Yo
Department Of Biochemistry Tokyo College Of Pharmacy
-
Hasumi Fujio
Department Of Biochemistry Tokyo College Of Pharmacy
-
TANJI NORIAKI
Department of Biochemistry, Tokyo College of Pharmacy
-
Tanji Noriaki
Department Of Biochemistry Tokyo College Of Pharmacy
関連論文
- LIMB DEFORMITY INDUCED IN CHICK EMBRYO BY HYDROXYUREA
- Comparative Studies on the Anticonvulsant Activity of Lipophilic Derivatives of γ-Aminobutyric Acid and 2-Pyrrolidinone in Mice
- PRODUCTION OF METALLOPROTEINASE IN GASTRIC TISSUE WITH ACETIC ACID-INDUCED ULCER OR TAUROCHOLIC ACIDINDUCED GASTRITIS
- Studies on Lipophilic Derivatives of γ-Aminobutyric Acid : N-Acyl Derivatives of 2-Pyrrolidone
- STIMULATION OF HYALURONIC ACID SYNTHESIS BY INSULIN-LIKE GROWTH FACTOR-I (IGF-I) IN RABBIT PERICARDIUM
- EFFECT OF COLCHICINE AND CYTOCHALASIN B ON THE SECRETION OF PROTEOCHONDROITIN SULFATE AND PROTEINFREE CHONDROITIN SULFATE SYNTHESIZED IN CARTILAGE SLICES
- EFFECT OF BLEOMYCIN, NEOCARZINOSTATIN, AND HYDROXYUREA ON THE PREFORMED CARRAGEENIN GRANULOMA IN RATS : MECHANISM OF HYALURONIC ACID ACCUMULATION BY BLEOMYCIN
- ALTERATIONS OF GLYCOSAMINOGLYCANS SYNTHESIZED BY CHICK EMBRYO CARTILAGE TREATED WITH 6-AMINONICOTINAMIDE
- PROSTAGLANDIN CYTOPROTECTION LINKED TO THE ADHESION OF MUCOUS GLYCOPROTEIN TO THE GASTRIC EPITHELIUM
- THE STUDIES ON THE ROLE OF GASTRIC GLYCOPROTEINS WITH REFERENCE TO CYTOPROTECTION : PROTECTIVE EFFECT OF PROSTAGLANDIN E_2 AND SOFALCONE ON ETHANOL-INDUCED NECROSIS
- Translational Augmentation of Pro-matrix Metalloproteinase 3 (Prostromelysin 1) and a Tissue Inhibitor of Metalloproteinases (TIMP)-1 mRNAs by Epidermal Growth Factor and Transforming Growth Factor α, but not by Interleukin 1α or 12-O-Tetradecanoylphorbol
- Effects of H_2-Receptor Antagonists on Matrix Metalloproteinases in Rat Gastric Tissues with Acetic Acid-Induced Ulcer
- Thyroid function in premature infants.
- Effects of Gonadal Hormones on Hyaluronic Acid Content in Male Mouse Skin
- CHANGES IN THE STRUCTURE OF RAT MUCOUS GLYCOPROTEIN AND PROSTAGLANDIN CYTOPROTECTION
- EFFECT OF WATER IMMERSION STRESS ON THE BIOSYNTHESIS OF RAT GASTRIC GLYCOPROTEINS WITH OR WITHOUT SULFATE
- CHANGES IN GLYCOSAMINOGLYCAN SYNTHESIS DURING THE DIFFERENTIATION OF HL-60 CELLS INDUCED BY 12-O-TETRADECANOYLPHORBOL-13-ACETATE
- 117 RELATIONSHIP BETWEEN VASCULAR ENDOTHELIAL CELL FUNCTION AND ADIPOCYTOKINES IN OBESE CHILDREN
- 66.STUDIES ON THE PHYSICAL FITNESS OF MARATHON AND LONG-DISTANCE RACERS. Report V.
- Design Aspects of an Ultrashort Ultraviolet Laser System
- EFFECTS OF DEHYDROEPIANDROSTERONE SULPHATE ON THE PRODUCTION OF COLLAGENASE AND GELATINOLYTIC METALLOPROTEINASE BY RABBIT UTERINE CERVICAL CELLS IN PRIMARY CULTURES
- Role of p-f hybridization in the metal-nonmetal transition of PrRu4P12
- Effect of Calcitonin on Experimental Osteolathyrism (III) Selective inhibition of collagem resorption by calcitonin
- Increase of Structural Glycoprotein during Dilatation of Human Cervix in Pregnancy at Term
- Effect of an anti-ulcer agent, 2'-carboxymethoxy-4,4'-bis(3-methyl-2-butenyloxy) chalcone (SU-88), on the biosynthesis of gastric sulfated mucosubstances in restrained and water-immersed rats.
- Changes in synthetic activity of sulfated mucosubstances in healing process of acetic acid ulcer in rats and effect of anti-ulcer agents.
- Cytoprotective effect of SU-88, an anti-ulcer agent, in the rat.
- Changes in the incorporating activity of 35S-sulfate into gastric sulfated glycoproteins in the rat with erosions by restraint and water immersion stress.
- Role of p-f hybridization in the metal-nonmetal transition of PrRu4P12
- The Incorporation Pattern of Glycine and Serine on the Biosynthesis of Collagen and Globin