CYTOPROTECTIVE EFFECT OF CYCLIC AMP ON CULTURED RABBIT GASTRIC CELLS
スポンサーリンク
概要
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A new experimental system with cultured gastric cells was developed to estimate the cytoprotective and damaging effects of drugs. The gastric cells were incubated in vitro in medium of pH 3,and their resistance to acid was determined by measruing percentage of damaged cells as a function of time. Pretretment with 0.5 mM acetylsalicylic acid (ASA) for 24 h decreased their resistance (26%), whereas pretreatment with 0.3-3 μg/ml of 16,16-dimethylprostaglandin E_2 (dimethyl PGE_2) for 4 h increased their resistance (13-16%). The adenosine 3' : 5'-cyclic monophosphate (cyclic AMP) level in the cultured gastric cells was decreased by 0.5 mM ASA (30%) and increased by 0.3 μg/ml of dimethyl PGE_2 (38%) on treatment for 24 h. These changes reflect phenomena observed in the gastric mucosa, suggesting that this experimental system is valid as an in vitro model. Concentrations of 0.1 mM cyclic AMP and 0.1 mM N^6,O^<2'>-dibutyryladenosine 3' : 5'-cyclic monophosphate (dibutyryl cyclic AMP) were found to exert cytoprotective effects on the cells (5 and 7% increase, respectively). Furthermore, 0.1 mM cyclic AMP caused partial recovery from 0.5 mM ASA-induced decrease in resistance (ASA : 17%, ASA+cyclic AMP : 9%). These findings suggest that cyclic AMP plays a role in protection of cultured gastric cells and presumably also of gastric mucosal cells in vivo.
- 公益社団法人日本薬学会の論文
著者
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Murota Sei-itsu
Department Of Pharmacology Tokyo Metropolitan Institute Of Gerontology
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Murota Sei-itsu
Department Of Cellular Physiological Chemistry Graduate School Tokyo Medical And Dental University
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MATUOKA KOOZI
Department of Pharmacology, Tokyo Metropolitan Institute of Gerontology
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MITUI YOUJI
Department of Pharmacology, Tokyo Metropolitan Institute of Gerontology
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Matuoka Koozi
Department Of Pharmacology Tokyo Metropolitan Institute Of Gerontology
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Mitui Youji
Department Of Pharmacology Tokyo Metropolitan Institute Of Gerontology
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