Effect of Polymethylene and Phenylene Linking Groups on the DNA Cleavage Specificity of Distamycin-Linked Hydroxamic Acid-Vanadyl Complexes

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概要

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• Two types of distamycin-linked hydroxamic acids (DHA), which contain various lengths of polymethylene chains (PM-DHA) and relatively rigid phenylene ones (Ph-DHA), have been synthesized for the first time. Their DNA cleavage specificities were investigated by an end-labeled fragment cleavage experiment in the presence of vanadyl ion and hydrogen peroxide. The DNA cleavage by the PM-DHA・VO(II) complexes was shown to be very dependent on the length of the chain and the AT sequences. The tetramethylene DHA (1b) complex exhibited highly specific cleavage patterns flanking the 8 and 10 AT sites. Interestingly, the Ph-DHA complexes selectively cleaved the 5' end-labeled strand at the AT sites, but did not cleave the 3' end-labeled strand. The vanadyl complexing moieties and the local sequence conformation of the AT tract are suggested to contribute significantly to the DNA recognition of the PM-DHA・VO(II) complexes.

• 公益社団法人日本薬学会の論文
• 2000-05-01

著者

• Hashimoto Shigeki

  Department Of Biological Science And Technology Science University Of Tokyo

• Nakamura Yushin

  Department Of Biological Science And Technology Science University Of Tokyo

• INUI Takahiro

  Department of Biological Science and Technology, Science University of Tokyo

• Inui Takahiro

  Department Of Biological Science And Technology Science University Of Tokyo

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• Effect of Polymethylene and Phenylene Linking Groups on the DNA Cleavage Specificity of Distamycin-Linked Hydroxamic Acid-Vanadyl Complexes
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