Disk to Sphere Transformation of Erythrocyte induced by 1-Anilino-8-Naphthalene Sulfonate (ANS). II. Quantitative Study on Adsorption of ANS by Whole Erythrocyte and by Ghost Membrane

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概要

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• Amounts of 1-anilino-8-naphthalene sulfonate (ANS) adsorbed by whole bovine erythrocyte and by its ghost membrane were studied at ANS concentration from 0.01 to 3mM. For whole erythrocyte the amount is nearly proportional to the free ANS concentration with the ratio of 1.1×10 [mole/l-packed-erythrocyte] / [mole/l-medium]. The ghost membrane has two kinds of binding site for ANS. The dissociation constants and the numbers of binding sites are calculated as follows : K_I=9.1×10^<-5>M and n_I=1.9×10^<-2> mole/kg-membrane for site I ; K_<II>=1.0×10^<-2> M and n_<II>=5.8×10^<-1> mole/kg-membrane for site II. The binding site I and II can be attributed to protein and lipid, respectively, judging from membrane mass per mole of sites. The mechanism of disk to sphere transformation was discussed from these results.

• 社団法人日本薬学会の論文
• 1976-12-25

著者

• 吉田 繁夫

  Department Of Physics Faculty Of Science University Of Tokyo

関連論文

• Disk to Sphere Transformation of Erythrocyte induced by 1-Anilino-8-Naphthalene Sulfonate (ANS). II. Quantitative Study on Adsorption of ANS by Whole Erythrocyte and by Ghost Membrane

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