The Use of a New Silylating Agent for Analysis of Catecholamines by Gas Chromatography-Mass Spectrometry
スポンサーリンク
概要
- 論文の詳細を見る
3-Methoxytyramine, dopamine, norepinephrine, epinephrine and 6-hydroxydopamine were derivatized to their N-trifluoroacetyl-O-dimethyl-n-propylsilyl ether or 2'-O-methyl-N-trifluoroacetyl-O-dimethyl-n-propylsilyl ether derivatives by treatment with trifluoroacetic anhydride, methanol and then dimethyl-n-propylsilyl imidazole. These derivatives could be separated completely by gas chromatography (GC) on a non-polar liquid stationary phase such as OV-101. In addition, these derivatives were very stable in benzene and ethyl acetate, and showed excellent stability during silica gel column chromatography in comparison with the corresponding trimethylsilyl ether derivatives. In GC-mass spectrometry (MS) of these derivatives, ammonia chemical ionization (CI)-MS provided the ion [M+NH_4]^+ as the base peak or an intense peak. The detection limit of the dopamine derivative in the CI mode with ammonia was 2 pg with S/N at 2 : 1.
- 公益社団法人日本薬学会の論文
- 1981-03-25
著者
-
石橋 正兀
Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.,
-
石橋 正兀
日本化薬(株)医薬事業本部総合研究所
-
宮崎 浩
寿製薬研究部
-
山下 幸和
Pharmaceutical Institute Tohoku University
-
山下 幸和
日本化薬(株)医薬事業本部総合研究所
-
宮崎 浩
Research Laboratories of Pharmaceuticals Group, Nippon Kayaku Co., Ltd.,
-
石橋 正兀
Research Laboratories Of Pharmaceuticals Group Nippon Kayaku Co. Ltd.
-
山下 幸和
Research Laboratories, Pharmaceutical Group, Nippon Kayaku Co.,
-
薬師寺 晟
Scientific Instrument Division, Dainiseikosha Co.
-
薬師寺 晟
Scientific Instrument Division Dainiseikosha Co.
-
山下 幸和
Research Laboratories Pharmaceutical Group Nippon Kayaku Co.
関連論文
- FEASIBILITY OF USING [^O_2]11-DEHYDROTHROMBOXANE B_2 AS AN INTERNAL STANDARD OF IMMUNOAFFINITY PURIFICATION FOLLOWED BY GAS CHROMATOGRAPHY/SELECTED ION MONITORING
- Dimethylisopropylsilyl Ether Derivative in Gas Chromatography/Mass Spectrometry of 11-Dehydrothromboxane B_2. II. : Fragmentatin of Methyl Ester-11-n-propylamide-dimethylisopropylsilyl Ether Derivative
- DIMETHYLISOPROPYLSILYL ETHER DERIVATIVE IN GAS CHROMATOGRAPHY/MASS SPECTROMETRY OF 2,3-DINOR-6-KETO-PROSTAGLANDIN F
- Diethylhydrogensilyl-Cyclic Diethylsilylene Derivatives in Gas Chromatography : Mass Spectrometry of Prostanoids. IV. : 11-Dehydrothromboxane B_2
- ガスクロマトグラフィー-マススペクトロメトリー(GC/MS)によるプロスタノイド分析のための Diethylhydrogensilyl-Cyclic Diethylsilylene (DEHS-DES) 誘導体 (第2報) : GC 及び GC/MS におけるトロンボキサンB_2 Methyl Ester-Methyloxime-DEHS-DES 誘導体の挙動
- THE DIETHYLHYDROGENSILYL-CYCLIC DIETHYLSILYLIVE DERIVATEVE IN THE GAS CHROMATOGRAPHY-MASS SPECTROMETRY OF THROMBOXANE B_2
- ガスクロマトグラフィー/セレクテッドイオンモニタリングによるアコニチン系アルカロイドの分析
- 名誉会員 Evan C. Horning 先生を悼む
- 37 シンジュの苦味成分
- 36 前胸腺によるエクダイソンの生合成
- Fusaric Acid(5-Butylpyridine-2-carboxylic Acid) と類似の構造を持つアゾールカルボン酸の代謝
- Fusaric Acid(5-Butylpyridine-2-carboxylic Acid) アザアナローグの代謝
- Isoxazole Derivatives as Centrally Acting Muscle Relaxants. II. : Synthesis and Structure-Activity Relationship of 3-Amino-N-(3-phenyl-5-isoxazolyl)propanamides
- Isoxazole Derivatives as Centrally Acting Muscle Relaxants. I. : Synthesis and Activity of 5-(3-Aminopropyl)amino-3-phenylisoxazole Derivatives
- Antivertigo Agents. V. Quantitative Structure-Activity Relationships of 6-[2-(4-Aryl-1-piperazinyl) ethyl]-5,6,7,8-tetrahydro-1,6-naphthyridines
- Antivertigo Agents. III. Synthesis of 5,6,7,8-Tetrahydro-1,6-naphthyridine Methyl Homologs
- Antivertigo Agents. II. Structure-Activity Relationships of 6-Substituted 5,6,7,8-Tetrahydro-1,6-naphthyridines
- Antivertigo Agents. I. Structure-Activity Relationships of 2-(2-Aminoethyl) pyridines
- Syntheses of Heteroaromatic Carboxylic Acids Closely Related to Fusaric Acid
- Studies on Pyrimidine Derivatives. XIX. Synthesis of Isoxazolo [5,4-d] pyrimidines
- Semisynthesis of ^C-Labelled Leupeptin
- ガスクロマトグラフィー/マススペクトロメトリーによる2型及び3型プロスタノイドの分離条件の検討とエイコサペンタエン酸(EPA)摂取により生成する3型プロスタノイドへの応用
- DIETHYLHYDROGENSILYL-CYCLIC DIETHYLSLYLENE DERIVATIVES IN GAS CHROMATOGRAPHY/MASS SPECTROMETRY OF HYDROXYLATED STEROIDS. V. : ANALYSIS OF CORTISOL AND 6β-HYDROXYCORTISOL IN HUMAN URINE
- Diethylhydrogensilyl Cyclic Diethylsilylene Derivatives in Gas Chromatography-Mass Spectrometry of Hydroxylated Steroids. II. : Pregnanes with a Hydroxylated 17β-Side-Chain
- 高速液体クロマトグラフィーによる生体試料中エトポシドの定量 : 自動前処理法の開発(医薬品の分析)
- Gas Chromatography-Selected Ion MonitoringによるFM-100投与後の血中グリチルリチン及びグリチルレチン酸の定量
- The Use of a New Silylating Agent for Analysis of Catecholamines by Gas Chromatography-Mass Spectrometry
- よいマススペクトルを得るには (GC-MSの医学・生化学への応用) -- (GC-MSの基礎と測定法)
- Analysis of Bile Acids and Cholesterol by Open Tubular Glass Capillary-Selected Ion Monitoring
- 75 人形峠ウラン鉱床出土植物化石の成分研究
- Use of New Silylating Agents for Identification of Hydroxylated Steroids by Gas Chromatography-Electron Impact-Mass Spectrometry
- Mass Fragmentographic Determination of trans-4-Aminomethylcyclohexanecarboxylic Acid (Tranexamic Acid) by Use of Peak Matching Operation
- Mass Fragmentographic Determination of d- and l-Chlorpheniramine with Aid of the Stable Isotope Technique
- Mass Fragmentographic Determination of 1-Piperidino-2,4'-dimethylpropiophenone (Mydocalm) by Use of 1 : 1 Mixture Technique
- Analysis of Bile Acids by Mass Fragmentography : Application of 1 : 1 Mixture Technique by Use of Stable Isotope labeled Compounds
- GC-MSによるプロスタグランジンおよびトロンボキサン類の分析 (バイオメディカルクロマトグラフィ--1-) -- (薬物および薬物代謝のクロマトグラフィ-)
- Preparation and Antigenic Properties of 5α-Dihydrotestosterone-15α-Bovine Serum Albumin Conjugate
- Preparation and Antigenic Properties of Testosterone-15α-Protein Conjugate
- Synthesis of 15α-Hydroxytestosterone and Related C_ Steroids
- Blockage of Coordination with Shift Reagent by tert-Butyldimethylsilylation in Nuclear Magnetic Resonance Spectroscopy and Its Applications to Deuterium-Labeled Steroids
- Synthesis of 15α-Hydroxylated Dehydroepiandrosterone and Androstenediol
- Stereospecific Introduction of Deuterium into Ring D of Estrogens
- Syntheses of 15α-Hydroxyestrone and 15α-Hydroxyestradiol
- Studies on Dimethyl-tert-butylsilyl Ethers of Steroid