Direct Injection Analysis of Atenolol Enantionmers in Plasma Using an Achiral/Chiral Coupled Columun HPLC System

概要

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A novel HPLC system was developed with an achiral/chiral coupled column for the direct injection analysis of atenolol (AT) enantiomers in plasma. The system consists of a size-exclusion column, an ODS silica column and a newly developed β-cyclodextrin perhenylcarbamate(ph-β-CD)-bonses silica column connected in a series via two switching valves. The neat plasma sample was directly injected onto the size-exclusion column, and the deproteinzed fraction of AT enantiomers was concentrated on the ODS silica column. The enantimers were then transrerred and separated mutually on the ph-β-CD silica column. The calibration line for each of the AT enantiomers was linear in the range of plasma concentration of 10-200 ng/ml (r>0.9997) with good reproducibility (CV<9.7%, n=20). The recoveries from plasma were almost complete (>97.4%) for both enatiomers. One analysis finished within 30 min. The developed system was applied to the enantioselective determination of plasma concentration-time curve of AT after the oral administration of racemic AT to a healthy volunteer.
社団法人日本薬学会の論文
1993-03-15