Deoxyribonucleic Acid Strand Break Induced by the Hydroxy Radical-Generating Cyclic Peroxide, 4-Ethoxy-1,4-dihydro-2,3-benzodioxin-1-ol

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Deoxyribonucleic acid (DNA) strand break caused by a synthetic cyclic peroxide, 4-ethoxy-1,4-dihydro-2,3-benzodioxin-1-ol (Bd) was studied by both ethidium bromide fluorescence quenching and agarose gel electrophoresis. The Bd-mediated DNA strand break occurred dependently on temperature and also on Bd concentration. The reaction proceeded at a temperature higher than 30℃ (decomposition temperature of Bd), indicating that the reactive species generated by Bd-decomposition are responsible for the reaction. The reaction was protected by 1,4-diazabicyclo[2.2.2]octane and NaN_3. and also moderately by OH radical scavengers such as Na-benzoate and NaBr. Possible involvement of active oxygen radicals including OH radical in the DNA strand break is discussed in relation to the mechanism of Bd decomposition.
公益社団法人日本薬学会の論文
1991-11-25