Encapsulation of Drugs by Lyophilized Empty Dipalmitoylphosphatidylcholine Liposomes : Effect of Calcium Ion

概要

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The effects of divalent cation (Ca^<2+>) on the characteristics of dipalmitoylphosphatidylcholine (DPPC) liposomes regenerated from lyophilized empty liposomes by rehydration and wariming were investigated. The results showed that the volume (ml) of internal aqueous compartment per g lipid (captured volume; V_<cap>) has a maximum at a certain concentration range of calcium chloride and the maximal value is than ten times the minimal value. This phenomenon can be explained by considering that binding of Ca^<2+> to phosphate groups in DPPC molecules induces an increase in the distance (r) between adjacent bilayer membranes in multilamellar liposomes through electrostatic force and causes an increase in V_<cap>. The dynamic properties of lyophilized liposomes in the rehydration process were examined using a multilamellar vesicle model. The results of simulation suggested that a repulsive force induced between the adjacent bilayer membranes causes rearragement of the constituent lipid molecules in a liposome followed by an increase in the distance r, a decrease in the internal lamellar number, a smaller increase in liposome size and finally a significant increase in V_<cap>.
公益社団法人日本薬学会の論文
1989-11-25