Interaction of Rhizopus delemar Lipase with 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane and Structurally Related Pesticides : Importance of 1 : 1 Pesticide-Lipase Complexes

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The interation of Rhizopus delemar C-lipase with 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) and structurally related pesticides was studied. Binding assays and titration experiments revealed that the binding is distinctly cooperative and the 1 : 1 pesticide-lipase complex formation governs the ensuing complex formation. The chlorinated pesticides used here fall into three groups according to the mode of interaction with lipase. (1) DDT, 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD) and 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE), the 1 : 1 pesticide-lipase complexes of which bind 8 further molecules of any pesticide used here to form 9 : 1 complexes with much higher activity. (2) Dimic and dichlorobenzophenone, the 1 : 1 pesticide-lipase complexes of which can bind 8 further molecules of the same pesticide without activation, but cannot bind any other pesticide. (3) Kelthane, chlorobenzilate and chloropropylate, which are non-competitive inhibitors of the intact lipase but behave as activators for 1 : 1 pesticide-lipase complexes of pesticides in group (1).The formation of the very tightly bound 9 : 1 DDT-lipase complex at pH 4.5 Chromatography on CM-Sephadex C25 gave results supporting the one-step formation of a 9 : 1 DDT-lipase complex from a 2 : 1 DDT-lipase complex.
社団法人日本薬学会の論文
1988-01-25