Determination of Free Fatty Acids in o/w Injectable Emulsions by HPLC with Fluorescence Detection Using 4-(N, N-Dimethylaminosulfonyl)-7-N-piperazino-2,1,3-benzoxadiazole

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概要

• 論文の詳細を見る

• HPLC using the fluorescent reagent, 4-(N, N-dimethylaminosulfonyl)-7-N-piperazino-2,1,3-benzoxadiazole (DBD-PZ) was applied to the determination of free fatty acids in an o/w injectable emulsion. This fluorescence HPLC method was highly sensitive and the free fatty acids could be directly derivatized without any previous preparation such as solvent extraction and elimination of water. In addition, the derivatized fatty acids could be adequately separated from interfering peaks, i.e., reagent and by-product peaks.The detection limit of this HPLC method was ca. 5 fmol in the case of C_<18 : 0> fatty acid. The mean recovery of 2.5 nmol of each fatty acid (C_<8 : 0>, C_<14 : 0>, C_<16 : 0>, C_<18 : 0>) added to the o/w injectable emulsion was 101.6% (n=3). This fluorescence HPLC method with DBD-PZ was applied to commercial nonaqueous injection preparations to study their stability, and each free fatty acid in these preparations could be determined successfully. These results indicate that the proposed fluorescence HPLC method can be adequately used for the determination of free fatty acids in an o/w injectable emulsion.

• 公益社団法人日本薬学会の論文
• 1999-10-15

著者

• NISHIJIMA Koji

  Drug Analysis & Pharmacokinetics Research Laboratories, Pharmaceutical Development Division, Takeda

• Nishijima Koji

  Drug Analysis And Pharmacokinetics Research Laboratories Pharmaceutical Research Division Takeda Che

• UENO Yumiko

  Drug Analysis and Pharmacokinetics Research Laboratories, Pharmaceutical Research Division, Takeda C

• MATSUNAGA Hirokazu

  Drug Analysis and Pharmacokinetics Research Laboratories, Pharmaceutical Research Division, Takeda C

• UMEMOTO Kazuichi

  Drug Analysis and Pharmacokinetics Research Laboratories, Pharmaceutical Research Division, Takeda C

• Ueno Y

  Drug Analysis And Pharmacokinetics Research Laboratories Pharmaceutical Research Division Takeda Che

• Ueno Yumiko

  Drug Analysis And Pharmacokinetics Research Laboratories Pharmaceutical Research Division Takeda Che

• Matsunaga Hirokazu

  Drug Analysis & Pharmacokinetics Research Laboratories Takeda Chemical Industries Ltd.

• Matsunaga Hirokazu

  Drug Analysis And Pharmacokinetics Research Laboratories Pharmaceutical Research Division Takeda Che

• Umemoto Kazuichi

  Drug Analysis And Pharmacokinetics Research Laboratories Pharmaceutical Research Division Takeda Che

関連論文

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• Determination of Free Fatty Acids in o/w Injectable Emulsions by HPLC with Fluorescence Detection Using 4-(N, N-Dimethylaminosulfonyl)-7-N-piperazino-2,1,3-benzoxadiazole
• Degradation Products Generated by Sonication of Benzyl Alcohol, a Sample Preparation Solvent for the Determination of Residual Solvents in Pharmaceutical Bulks, on Capillary Gas Chromatography
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