Poly(ethylene glycol) Derivative of Cholestrol Reduces Binding Step of Liposome Uptake by Murine Macrophage-like Cell Line J774 and Human Hepatoma Cell Line HepG2
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概要
- 論文の詳細を見る
Liposome uptake by HepG2 human hepatoma cells was investigated in comparison with the uptake by J774 murine macrophage-like cells. HepG2 cells accumulated liposomes (egg yolk phosphatidylcholine (EPC)/Chol; 75/25,diameter 0.2 μm) at 37℃ comparably to J774 macrophage-like cells. Confocal microscopic observations revealed that J774 cells internalized EPC/Chol liposomes efficiently but HepG2 cells kept most or the liposomes bound on their plasma membrane surfaces. Poly(ethylene glycol) (PEG)-coated liposomes (0.2 μm) containing poly(ethylene glycol) cholesteryl ether (PEG-Chol) avoided cellular uptake at 37℃ by either cell line. In both cell lines, binding of PEG-coated liposomes was lower than that of EPC/Chol liposomes when incubation was carried out at 4℃. To analyze the binding process at 37℃, surface-bound liposomes were removed from the cells by pronase treatment. A reduction of the amount of bound-liposomes on cell surfaces was observed in the case of PEG-coated liposomes. Therefore, PEG/coating reduces direct binding of liposomes to the cell surfaces. The presence of apolipoprotein E (apoE) increased the uptake of EPC/Chol liposomes via its receptor in both cell lines. In contrast, cellular uptake of PEG-coated liposomes was not enhanced by treatment with apoE. Therefore, while apoE-mediated liposome uptake occurs in the case of EPC/Chol liposomes, it does not occur for PEAG-coated liposomes; PEG-coating also inhibits protein-mediated binding to the cells. These results further imply that elusion from liver clearance of PEG-coated liposomes is not only due to the reduction of uptake by Kupffer cells but also by hepatocytes when liposomes are small enough to go through the fenestrates of the endothelial lining.
- 公益社団法人日本薬学会の論文
- 1998-12-15
著者
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Oku Masahide
Department Of Biophysics Graduate School Of Science Kyoto University
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SATO Satoshi
Department of Pediatrics, Tokyo Medical University
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Kobayashi Shoichi
Laboratory Of Food And Health Science Department Of Agro-bioscience Faculty Of Agriculture Iwate Uni
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ISHIWATA Hideki
Department of Physical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
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KOBAYASHI Satoe
Department of Physical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
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VERTUT-DOI Aline
Department of Physical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
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MIYAJIMA Koichiro
Department of Physical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
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Ishiwata H
Department Of Physical Chemistry Faculty Of Pharmaceutical Sciences Kyoto University:(present Adress
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Vertut‐doie A
Department Of Physical Chemistry Faculty Of Pharmaceutical Sciences Kyoto University
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Miyajima Koichiro
Department Of Physical Chemistry Faculty Of Pharmaceutical Sciences Kyoto University
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Sato Satoshi
Department Of Pediatrics Yamagata University School Of Medicine
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Kobayashi Satoe
Department Of Physical Chemistry Faculty Of Pharmaceutical Sciences Kyoto University
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Sato Satoshi
Department Of Biophysics Graduate School Of Science Kyoto University
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Sato Satoshi
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Chiba University
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