Determination of the Diffusion Coefficient of Insulin and Lysozyme in Crosslinked Dextran Hydrogels by Pulsed-Field-Gradient NMR
スポンサーリンク
概要
- 論文の詳細を見る
Crosslinked dextran hydrogels were prepared from glycidyl methacrylate (GMA)-derivatized dextran by gammma-irradiation initiation in the presence of insulin and lysozyme as model protein and peptide drugs. The diffusion coefficient of insulin and lysozyme in the hydrogels was determined sucessfully by a pulsed-field-gradient stimulated-echo method. A conventional NMR probe which can impose a relatively small magnetic field gradient (30 Gauss/cm) was used for the measurements. This method seems to be applicable to proteins and peptides with a molecular weight of about 10000 or less. The diffusion coefficient determined by the NMR method almost agreed with that calculated from the drug release profiles, indicating the usefulness of the diffusion coefficient determined by the NMR method as a measure of the release rate from hydrogels.
- 公益社団法人日本薬学会の論文
- 1998-11-15
著者
-
Kojima Shigeo
National Institute Of Health Sciences
-
YOSHIOKA SUMIE
National institute of Hygienic Sciences
-
Yoshioka Sumie
National Institute Of Health Sciences
-
ASO Yukio
National Institute of Health Sciences
-
ASO Yukiko
National Institute of Health Sciences
関連論文
- DEPENDENCE OF THE SOLID-STATE HYDROLYSIS AND OXIDATION RATE OF DRUGS ON TEMPERATURE AND HUMIDITY
- Abnormal Dissolutions of Chlorpromazine Hydrochloride Tablets in Water by Paddle Method under a High Agitation Condition
- Mass Variation Tests for Coating Tablets and Hard Capsules : Rational Application of Mass Variation Tests
- The Study of the Applicability of Content Uniformity and Weight Variation Test : The State of Commercial Tablets and Capsules in Japan
- Effects of Codeine on the Agitating Force and Gastrointestinal Transit Time in Dogs, for Use in Drug Absorption Studies
- Effects of Atropine and Loperamide on the Agitating Force and GI Transit Time in Dogs in Drug Absorption Studies
- Pharmacognostical Studies of Cistanchis Herba(III)Phylogenetic Relationship of the Cistanche Plants Based on Plastid rps2 Gene and rpl16-rpl14 Intergenic Spacer Sequences
- Miscibility of Nifedipine and Hydrophilic Polymers as Measured by ^1H-NMR Spin-Lattice Relaxation
- Effect of Water on the Molecular Mobility of Sucrose and Poly(vinylpyrrolidone) in a Colyophilized Formulation as Measured by ^C-NMR Relaxation Time
- Feasibility of ^F-NMR for Assessing the Molecular Mobility of Flufenamic Acid in Solid Dispersions
- Physical Stability of Amorphous Acetanilide Derivatives Improved by Polymer Excipients
- Comparison of the Glass Transition Temperature and Fragility Parameter of Isomalto-Olygomer Predicted by Molecular Dynamics Simulations with Those Measured by Differential Scanning Calorimetry
- Effect of Freezing Rate on Physical Stability of Lyophilized Cationic Liposomes
- Usefulness and Limitations of Nonisothermal Prediction of Drug Stability
- Molecular Mobility of Lyophilized Poly(vinylpyrrolidone) and Methylcellulose as Determined by the Laboratory and Rotating Frame Spin-Lattice Relaxation Times of ^1H and ^C
- A Comparison of the Analysis of Covariance (ANCOVA) and Range-Based Approaches for Assessing Batch-to-Batch Variability of the Stability of Pharmaceutical Products
- Effect of Polymer Excipients on the Enzyme Activity of Lyophilized Bilirubin Oxidase and β-Galactosidase Formulations
- Solid-State Rehydration-Induced Recovery of Bilirubin Oxidase Activity in Lyophilized Formulations Reduced during Freeze-Drying
- Assessment of Shelf-Life Equivalence of Pharmaceutical Products
- Power of Analysis of Variance for Assessing Batch-Variation of Stability Data of Pharmaceuticals
- Relationship between Water Mobility, Measured as Nuclear Magnetic Relaxation Time, and the Crystallization Rate of Amorphous Nifedipine in the Presence of Some Pharmaceutical Excipients
- Determination of the Diffusion Coefficient of Insulin and Lysozyme in Crosslinked Dextran Hydrogels by Pulsed-Field-Gradient NMR
- Assessment of Gastric Acidity of Japanese Subjects over the Last 15 Years
- Accumulation of NCC-CO-411 Monoclonal Antibody in Human Colorectal Adenocarcinoma through Binding of the Released Carcinoembryonic Antigen in Nude Mice
- Enhancement of the Antitumor Effect of γ-Ray Irradiation in Combination with Camptothecin against Human Colorectal Adenocarcinoma
- Protection of Protein Secondary Structure by Saccharides of Different Molecular Weights during Freeze-Drying
- Effect of Sodium Tetraborate (Borax) on the Thermal Properties of Frozen Aqueous Sugar and Polyol Solutions
- Pharmacognostical Studies of Cistanchis Herba (IV) Phylogenetic Relationship of the Cistanche Plants Based on Plastid rps2 Gene and rpl16-rpl14 Intergenic Spacer Region Sequence