An Experimental Approach to Study the Binding Properties of Vitamin E(α-Tocopherol) during Hairless Mouse Skin Permeation

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概要

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• An experimental approach to study the binding properties of vitamin E has been developed. Total vitamin E solubility in the skin was determined by a partition study, followed by in vitro skin permeation studies with whole skin and stripped skin. The amount of freely diffusable vitamin E in the diffusion process was determined from the permeation profiles of whole skin and stripped skin by employing a bi-layer model. The concentrations of vitamin E in the stratum corneum and viable dermis were determined separately. By subtracting this amount from the total concentration of vitamin E in the skin, as determined by the solubility study, the amount of bound vitamin E was determined. After skin permeation reached a steady state, the donor solution was removed and the permeation study continued (desorption study). During the entire period of the desorption experiment, the amount of vitamin E in the receptor solution hardly increased and remained constant. After the desorption experiment, vitamin E still remaining in the skin was determined by extracting with tissue solubilizer, SOLABLE, and is considered as the amount of vitamin E strongly bound in the skin. The concentrations of bound vitamin E determined by permeation and desorption studies coincided relatively well. To further investigate skin binding of vitamin E, a differential scanning calorimetry study was performed. Vitamin E-treated stratum corneum showed phase transitions at 76 and 85℃, associated with lipid transitions. The thermal transitions associated with the lipid transition suggested interactions of vitamin E with lipid components of the skin. During skin permeation, vitamin E forms a very strong reservoir in the skin tissue and this amount of vitamin E, about 30%, exists as a bound-form with the lipid components of the stratum corneum.

• 公益社団法人日本薬学会の論文
• 2001-06-01

著者

• Lee A‐r

  College Of Pharmacy Duksung Women's University

• Lee Ae-ri

  韓国

• Tojo Kakuji

  Faculty Of Computer Science And Systems Engineering Kyushu Institute Of Technology

• LEE Ae-Ri

  College of Pharmacy, Duksung Women's University

• LEE Ae-Ri

  College of Pharmacy, Duksung Women's University

関連論文

• An Experimental Approach to Study the Binding Properties of Vitamin E(α-Tocopherol) during Hairless Mouse Skin Permeation
• Control of the Barrier Capacity of Stratum Corneum by Microdermabrasion
• Synergistic Effect of Sonophoresis and Iontophoresis in Transdermal Drug Delivery
• Mechanisms of Synergistic Skin Penetration by Sonophoresis and Iontophoresis
• A Pharmacokinetic Model for Ocular Drug Delivery
• Effects of Pathological Conditions on Ocular Pharmacokinetics of Antimicrobial Drugs

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