Hypochlorous Acid Inhibition by Acetoacetate : Implications on Neutrophil Functions(Biochemistry/Molecular Biology)
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概要
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Type-1 diabetic patients experience hyperketonemia caused by an increase in fatty acid metabolism. Thus, the aim of this study was to measure the effect of ketone bodies as suppressors of oxidizing species produced by stimulated neutrophils. Both acetoacetate and 3-hydroxybutyrate have suppressive effect on the respiratory burst measured by luminol-enhanced chemiluminescence. Through measurements of hypochlorous acid production, using neutrophils or the myeloperoxidase/H_2O_2/Cl^- system, it was found that acetoacetate but not 3-hydroxybutyrate is able to inhibit the generation of this antimicrobial oxidant. The superoxide anion scavenging properties were confirmed by ferricytochrome C reduction and lucigenin-enhanced chemiluminescence assays. However, ketone bodies did not alter the rate of oxygen uptake by stimulated neutrophils, measured with an oxygen electrode. A strong inhibition of the expression of the cytokine IL-8 by cultured neutrophils was also observed ; this is discussed with reference to the antioxidant-like property of acetoacetate.
- 公益社団法人日本薬学会の論文
- 2004-08-01
著者
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Costa Miriani
Department Of Clinical Analysis (lac) State University Of Sao Paulo (unesp) School Of Pharmaceutical
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XIMENES Valdecir
Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, UNESP-Sao Paulo State Universit
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FONSECA Luiz
Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, UNESP-Sao Paulo State Universit
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Fonseca Luiz
Department Of Clinical Analysis (lac) State University Of Sao Paulo (unesp) School Of Pharmaceutical
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Ximenes Valdecir
Department Of Clinical Analysis Faculty Of Pharmaceutical Sciences Unesp-sao Paulo State University
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Da Fonseca
Department Of Pediatric Surgery Federal University Of Sao Paulo Escola Paulista De Medicina
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Da Costa
Departamento De Biologia Centro De Ciencias Universidade Federal Do Ceara
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XIMENES Valdecir
Department of Clinical Analysis (LAC), State University of Sao Paulo (UNESP), School of Pharmaceutical Science
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