Asialoganglioside Enhances the Efficiency of Gene Transfection Mediated by Cationic Liposomes with a Cationic Cholesterol Derivative(Biopharmacy)

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We investigated the transfection efficiency mediated by asialoganglioside-containing cationic liposomes. Previously we reported that monosialoganglioside GM_1 (GM_<1a>) enhanced transfection efficiency. In this study, we investigated the effects of sialic acid in gangliosides on transfection efficiency. Two mammalian culture cell lines HeLa and HepG2 were transfected with luciferase plasmids (pGL3) using cationic liposomes which contain monosialoganglioside GM_1 (GM_<1a>) or its asialic counterpart, asialoganglioside GM_1 (GA_1). Both GM_<1a> and GA_1 enhanced the efficiency of transfection mediated by cationic liposomes, and GA_1 exhibited higher efficiency than GM_<1a> in both cell lines. Transfection efficiency of ganglioside-containing liposomes was also assessed by the effects of antisense oligonucleotides (AS-ODN) for bcl-2 gene, which suppresses apoptotic cell death. Western blotting analysis revealed that the expression of Bcl-2 was decreased by AS-ODN, and the reduction of protein expression in cells treated with GA_1-containing liposomes was more remarkable than that with GM_<1a>-containing liposomes. Furthermore, the induction rate of apoptosis was higher in cells treated with AS-ODN with GA_1-containing liposomes. Together with the results obtained by luciferase assay mentioned above, the removal of sialic acid from ganglioside causes the enhancement of efficiency of transfection mediated by cationic liposomes.
公益社団法人日本薬学会の論文
2003-09-01

Asialoganglioside Enhances the Efficiency of Gene Transfection Mediated by Cationic Liposomes with a Cationic Cholesterol Derivative(Biopharmacy)

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