Immunohistochemical Study of Vasopressin and Oxytocin in the Neurosecretory System during Reorganization of the Neural Lobe in Mice(Endocrinology)

概要

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Male mice of the C57BL/6NCrj strain were hypophysectomized at 3 months of age, and the changes in the neurosecretory system were studied immunohistochemically using anti-vasopressin (AVP) and anti-oxytocin (OXT) sera. In hypophysectomized mice, AVP and OXT fibers markedly increased at the cut of the infundibular stalk and in the external layer of the median eminence. The reorganized neural lobe (NL)-like tissue 20 days after hypophysectomy was strongly stained with anti-AVP and anti-OXT sera. However, the extent of stainability was not different between the two kinds of antisera. Thirty days after hypophysectomy, the stainability to anti-AVP serum was far less than that to anti-OXT serum in the reorganized NL, suggesting that AVP was selectively released from the reorganized NL. AVP neurons in the principal region of the supraoptic (SON) and paraventricular nuclei (PVN) showed a marked deposition of immunoreactive AVP 5 days after hypophysectomy. At 30 postoperative days, AVP neurons in the SON showed further deposition of the antigen in their perikarya, while the accumulation of immunoreactive AVP in the PVN was very variable among AVP neurons. The perikarya of OXT neurons 5 days after hypophysectomy were strongly stained with anti-OXT serum. At 30 postoperative days the immunoreactivity of OXT neurons in both nuclei were not significantly different from that of the controls.
社団法人日本動物学会の論文
1985-06-15