Development of the Myotomal Neuromuscular System in Embryonic and Larval Angelfish, Pterophyllum scalare(Developmental Biology)
スポンサーリンク
概要
- 論文の詳細を見る
To reveal the developmental sequence of the myotomal neuromuscular system in a teleost angelfish, Pterophyllum scalare, we investigated the differentiation and axonal outgrowth of the somatic spinal motoneurons as well as the differentiation of the axial muscles by means of anatomical and histochemical methods. Acetylcholinesterase histochemistry and retrograde labeling with HRP revealed two large motoneurons in each spinal hemisegment in the late embryos. To clarify the posthatching change of the motoneuron number, the number of axons in the anal-level ventral root was counted, since ChAT-immunohistochemical labeling of cholinergic spinal neurons and electron microscopic observation in the adult showed that the ventral roots around the anal level contained only somatic motor axons. We found that 15 primary motoneurons in each spinal hemisegment participated in the muscle innervation in just-hatched larvae. The motor axons rapidly increased in number beyond the adult level within three days posthatching, and then decreased to reach the adult level within a few weeks. The result suggests that competition among the motoneurons for their target muscles takes place. To reveal the temporal sequence of differentiation of the myotomal muscle fibers, in addition to electron microscopic observation of the muscle, a fluorescent mitochondrial marker dimethylaminostyrylethylpyridiniumiodine (DASPEI) was used to detect red muscle fibers. In the late embryo, immature white muscle fibers subserving the twitching movement of the animal in the egg capsule were observed. Differentiation of the red muscle was not evident until day 10. The present results show that a complete set of the axial muscle motoneurons differentiates before the differentiation of the multiple muscle fiber types in the angelfish.
- 社団法人日本動物学会の論文
- 1999-10-15
著者
-
Sakamoto Tatsuya
Laboratory Of Physiology Faculty Of Integrated Arts And Sciences Hiroshima University
-
SAKAMOTO Hirotaka
Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima University
-
Uematsu Kazumasa
Laboratory Of Fish Physiology Granduate School Of Biosphere Sciences
-
Uematsu Kazumasa
Laboratory Of Fish Physiology Faculty Of Applied Biological Science Hiroshima University
-
Yoshida Masayuki
Laboratory Of Fish Physiology Granduate School Of Biosphere Sciences
-
Yoshida Masayuki
Laboratory Of Fish Physiology Faculty Of Applied Biological Science Hiroshima University
-
Sakamoto Hirotaka
Laboratory Of Brain Science Faculty Of Integrated Arts And Sciences Hiroshima University
-
Sakamoto Hirotaka
Laboratory Of Fish Physiology Faculty Of Applied Biological Science Hiroshima University:(present Ad
関連論文
- Histological Study on the Autonomic Innervation of the Stomach and Ovaries of the Nile Tilapia Oreochromis niloticus
- MODE OF ACTION AND FUNCTIONAL SIGNIFICANCE OF ESTROGEN INDUCING THE GROWTH OF CEREBELLAR PURKINJE CELLS(Physiology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- ANALYSIS OF THE ROLE OF ESTROGEN IN THE GROWTH OF CEREBELLAR PURKINJE CELLS USING AROMATASE-DEFICIENT MICE
- THE MODE OF ESTROGEN ACTION ON THE GROWTH OF PURKINJE CELLS(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Organizing actions of neurosteroids in the Purkinje neuron
- EXPRESSION AND LOCALIZATION OF THE PUTATIVE MEMBRANE PROGESTERONE RECEPTOR 25-DX IN THE DEVELOPING PURKINJE CELL(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- ENDCRINE DISRUPTING CHEMICALS PROMOTE PURKINJE DENDRITIC GROWTH DURING CEREBELLAR CORTICAL FORMATION(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- SEX STEROIDS SYNTHESIZED DE NOVO IN THE DEVELOPING PURKINJE NEURON PROMOTE DENDRITIC GROWTH DURING CEREBELLAR CORTICAL FORMATION
- ACTIVITY AND LOCALIZATION OF 3β-HYDROXYSTEROID DEHYDROGENASE/Δ^5-Δ^4- ISOMERASE IN THE ZEBRAFISH BRAIN
- Novel brain function:biosynthesis and actions of neurosteroids in neurons
- Developmental Changes in the Swimming Behavior and Underlying Motoneuron Activity in the Larval Angelfish, Pterophyllum scalare
- Development of the Myotomal Neuromuscular System in Embryonic and Larval Angelfish, Pterophyllum scalare(Developmental Biology)
- ACTUAL AND EFFECTIVE UTILIZATION OF NEURON SPECIFIC mRNAs TO IDENTIFY PARTICULAR NEURON GROUPS PARTICIPATING IN PARTICULAR FISH BEHAVIOUR(Physiology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Activities of mesencephalic nucleus neurons during fictive swimming of the carp Cyprinus carpio
- Novel [^3H]Clonidine Binding Sites in the Intestine of the Eel Acclimated to Sea Water
- Post-Hatching Development of the Brain in Octopus ocellatus(Developmental Biology)
- Recording cerebellar neuron activities in swimming goldfish
- A new method for recording activities of spinal motoneurons during fictive swimming in paralyzed teleost fish
- Spinal Motor and Dorsal Root Ganglionic Neurons in the Glass Eels of the Japanese Eel Anguilla japonica