Molecular Stability and Function of Mouse Hemoglobins(Biochemistry)
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概要
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The hemoglobin types of mouse strains can be distinguished according to patterns observed on cellulose acetate electrophoresis. The two common mouse hemoglobin patterns are single and diffuse. The differences in the patterns result from differences in the β-globin chains of the hemoglobin molecules. Mice with the single hemoglobin pattern have one β-globin type identified as β-single (Hbb^s), whereas mice with the diffuse hemoglobin pattern have two different β-globin types identified as β-major (Hbb^<maj>) and β-minor (Hbb^<min>). We examined the functional and stability properties in these mouse hemoglobins, and the oxygen binding properties of red blood cells obtained from mice with four different hemoglobin phenotypes: Hbb^s/Hbb^s, Hbb^s/Hbb^<min>, Hbb^<miln>/Hbb^<min> and Hbb^<maj>/Hbb^<min>. The P_<50>, the partial pressure of oxygen at which hemoglobin is half-saturated, of purified forms of Hbb^s, Hbb^<min> and Hbb^<maj> was 14.8±0.4mm Hg, 13.3±0.6mm Hg and 13.6±0.5mm Hg, respectively. The n value, determined from the slope of the Hill plot was 2.45 to 2.59 for the mouse hemoglobins. The alkaline Bohr effects of purified Hbb^s, Hbb^<min> and Hbb^<maj> were 0.69, 0.61 and 0.60, respectively. The mechanical stability of Hbb^s, Hbb^<min> and Hbb^<maj>, expressed by the first order kinetic constant, were 0.098±0.01/min, 0.027±0.013/min and 0.27/min, respectively. The P_<50> of red blood cell suspensions from lines of mice expressing Hbb^s/Hbb^s, Hbb^<min>/Hbb^<min>, Hbb^s/Hbb^<min> and Hbb^<maj>/Hbb^<min> were 40.2±1.8mm Hg, 40.4±1.5mm Hg, 38.9±1.4mm Hg, and 38.7±0.9mm Hg, respectively.
- 1998-10-15
著者
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Uchida K
Graduate School Of Information Sciences Tohoku University
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Reilly Michael
Division Of Hematology Department Of Pediatrics The Children's Hospital Of Philadelphia Univers
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UCHIDA Kou
Division of Hematology, Department of Pediatrics, The Children's Hospital of Philadelphia, Universit
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ASAKURA Toshio
Division of Hematology, Department of Pediatrics, The Children's Hospital of Philadelphia, Universit
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Asakura Toshio
Division Of Hematology Department Of Pediatrics The Children's Hospital Of Philadelphia Univers
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