トノサマガエル皮膚の螢光物質
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概要
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Three different fluorescent substances, named Rana-chrome 1, Rana-chrome 3 and Rana-chrome 4 have been obtained from the trichloroacetic acid extract of the frog skin (Rana nigromaculata). These were treated with mercuric acetate, adsorbed on charcoal and chromatographed from Dewex 1 formate by the elution with formic acid. Each fraction was rechromatographed on Dowex 50H^+ by gradient elution with hydrochloric acid respectively, and further purification was carried out on Dowex 1 formate by using ammonium formate as eluant. Rana-chrome 1 was identified as biopterin with due regard to elementary analyses, ultraviolet and infrared absorption spectra, molecular weight and degradation studies with permanganate and periodate oxidation. Rana-chrome 3, an unknown blue fluorescent compound, with a very similar ultraviolet sbsorption but with slightly different infrared spectrum from Rana-chrome 1, gave an empirical formula of C_7H_7O_3N_5 and a molecular weight of 210-220. This compound was not oxidized by periodate, and 2, 4-dinitrophenylhydrazon formation was also negative. Its permanganate oxidation product was identified as 2-amino-6-hydroxypteridine-8-carboxylic acid. Rana-chrome 4, a purple fluorescent substance, was not identical with isoxanthopterin in respect of an elementary analysis value, but its ultraviolet and infrared spectra closely resembled those of synthetic specimen. Rana-chrome 5 was isolated in small amounts and characterized, although its systematic method of purification has not yet completely been established, being always accompanied by the large amounts of guanin.
- 1959-08-15